Rigorous analysis of clustering shows that 60 genes were coexpressed (AKT1, BAD, BAX, BCL2, BDNF, CASP3, CASP8, CASP9, MYC, PIK3CD, MAPK1, MAPK10, and CYCS).
Genes AKT1, BAD, BAX, BCL2, CASP3, CASP8, CASP9, MYC, PIK3CD, MAPK1, MAPK10, and CYCS are commonly expressed in the cluster of colorectal cancer, neuronal signaling pathway, neuronal death, amytrophic lateral aclerosis, and tuberculosis .
While other proteins (GSK3B, POU5F1, MAPK14, CREB1, SOX2, KLF4, PRKACA, MAPK10, STAT1, ACTB, TUBB3, MYC, GAPDH, AKT1, and CTNNB1) are related with process of aging, neuronal diseases, cardiovascular diseases, abnormal brain development, mental retardation, schizophrenia, and mycobacterial and viral infections [60-62].
The significant highest FC in the older cockerels was for MAPK10 genes (see Figure S3).
The gene that showed the highest age-dependent change in expression level was MAPK10, which encodes a protein that participates in important signalling pathways such as MAPK, Toll-like receptor, TNF, and GnRH signalling pathways and that is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development .
These genes are Akt1, C3ar1, Ccl19, Ccr1, Cd97, Cxcl1, Fyb, Ifi202b, Ifi203, Ifi204, Ifi205, Ikbke, Lyz1, Map3k13, Mapk10
, Mapk13, Pln, Ppt1, Socs1, and Xiap.
Some drug targets are related to MAPK activation, such as MAP2K4, TLR4, MAPK10, MAPK14, NOX4, ADAR2A, CHRNA7, ERBB2, FLT3, HTR2A, and LPAR3.
In addition, the abovementioned ingredients interacted with TLR4, ALOX5, ALOX12, and MAPK10, all of which are critical for the inflammatory process.