We found that the 3' UTR segment of human MAPK1 gene contains a sequence that is complementary to nucleotides of hsa-miR-1229-5p (Figure 4A).
By using luciferase dual reporter assay, MAPK1 was identified as a new target of miR-1229-5p.
NANOG (restricted to ICM), SOX2 (restricted to ICM), CDH1, HAND1, and MAPK1
(functions on cell differentiation and TE development) were downregulated in the 5% [O.sub.2] group.
miR-28-5p target Tumor type Reference p21 Choriocarcinoma cells  MPL Myeloproliferative neoplasms  N4BP1 Myeloproliferative neoplasms/  ovarian cancer OTUB1 Myeloproliferative neoplasms  TEX-261 Myeloproliferative neoplasms  MAPK1
Myeloproliferative neoplasms  E2F6 Myeloproliferative neoplasms  MAD2L1 B-cell lymphomas  BAG1 B-cell lymphomas  RAP1B B-cell lymphomas/renal cell [3, 5] carcinoma
The enriched functions for genes in two downregulated modules (d-1 and d-5) with higher enrichment scores showed that genes in module d-1 (e.g., MAPK1, MAPK14, MITF, RAF1, JAK1, HBEGF, ROS1, and STAT3) were related to cell surface receptor linked signal transduction (P=1.71E-03), and enzyme-linked receptor protein signaling pathway (P=2.02E-03); while genes in module d-5 (e.g., PIP5K1B, PIP5K1C, PIP4K2B, CXCL12, and FN1) were mainly enriched in phosphatidylinositol metabolic processes (P=4.24E-04), glycerolipid metabolic processes (P=1.88E-03), and cell morphogenesis (P=2.06E-02; Table 4).
Five pathways were enriched for genes in the module d-1 (MAPK1, MITF, RAF1, JAK1, and STAT3): pancreatic cancer (P=5.47E-04), melanoma (P=8.52E-03), melanogenesis (p=1.48E-02), acute myeloid leukemia (P=7.67E-03), and cancer pathways (P=3.69E-03).
The regions studied are close to MDM2 (12q15), LIPC (15q21.1), and MAPK1
Interestingly, miR-200a-3p has a direct inhibitory action on MAPK1
Ortego-Centeno et al., "Altered AKT1 and MAPK1
gene expression on peripheral blood mononuclear cells and correlation with T-helper-transcription factors in systemic lupus erythematosus patients," Mediators of Inflammation, vol.
MafA levels in beta-cells might be regulated by posttranscriptional mechanisms, such as the phosphorylation of two residues (serines 14 and 65) located in the transcriptional activating domain by the mitogen-activated protein kinase 1 (also known as MAPK1
However, all major downstream signaling pathways of EGFR, such as AKT serine/threonine kinase (AKT)--mechanistic target of rapamycin (mTOR) pathway , Janus kinase (JAK)--signal transducer and activator of transcription (STAT) pathway , or mitogen-activated protein kinase 1 (MAPK1
) pathway , are reported to induce PD-L1 expression.
Through coexpression network construction and analysis of node degree, we found some functional and high connected hubs varied in EC and HIP region, such as CHRM1, MAPK1
, TGFBR1, LIFR, ERBB4, ERBIN, ATP5C1, IGF1R, GJA1, TJP1, AP2M1, CDK5, FGF2, TUBB, SLC22A3, DBT, CDK13, NLN, and MIF.