In addition, mitogen-activated protein 3 kinase 7 (MAP3K7), also named transforming growth factor (TGF-[beta])-activated kinase-1, is a serine/ threonine kinase (19).
In the present study, we compared the levels of miR-143, ERK5, and MAP3K7 in BC tissues, noncancerous tissues and normal breast tissues.
To investigate the relationship of miR-143 and ERK5, MAP3K7 or cyclin D1, cells were co-transfected with miR-143 mimic/inhibitor and small interfering RNA targeting (si)-ERK5, si-MAP3K7 or si-cyclin D1 (100 nm, Qiagen).
Then, the levels of miR-143, ERK5 and MAP3K7 were detected using SYBR[R] Premix Ex Taq[TM] (TaKaRa, Japan).
Next, sections were blocked with 1% serum, and then incubated with rabbit anti-human ERK5, phospho-ERK5 (p-ERK5), MAP3K7 or phospho-MAP3K7 (p-MAP3K7) polyclonal antibody (1:50, Santa Cruz, USA) at 4[degrees]C overnight.
The membranes were probed with mouse anti-phospho-ERK5 (p-ERK5), ERK5, p-MAP3K7, MAP3K7 and cyclin D1 polyclonal antibodies (1:500, Santa Cruz) and mouse anti-GAPDH monoclonal antibody (1:2000, Sigma) overnight at 4[degrees]C, respectively.
Pearson correlation analysis was used to evaluate the correlation of miR-143 with ERK5 or MAP3K7. P<0.05 was considered to be significant.
Relationship of miR-143 and ERK5 or MAP3K7 in BC tissues
Among them, HDAC9, MCM3AP, SPIB, WT1, ethylmalonic encephalopathy 1 (ETHE1), BH3 interacting domain death agonist (BID), superoxide dismutase 2 (SOD2), and mitogen-activated protein kinase kinase kinase 7 (MAP3K7) genes are known to be involved in hepatocarcinogenesis, apoptosis, and anti-oxidative pathways, and their expressions are directly regulated by NF-[kappa]B.
MAP3K7 is linked to IL1 receptor and tumor necrosis factor (TNF) receptor signalings (Sato et al.
Dominant-negative MAP3K7 induces GO exit in the quiescent liver and accelerates hepatic cell cycle progression during regeneration (Bradham et al.