MAP3K3

MAP3K3

A gene on chromosome 17q23.3 that encodes a MAPK kinase kinase belonging to a protein kinase signal transduction cascade. MAP3K3 activates NF-kappaB, AP1 and DDIT3 transcription regulators.

Each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of three protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which in turn activates a specific MAPK.
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Forward (F) and reverse (R) primers for MAP3K3 (XM_015299375, F: 5'-TGA CCC GGA AAT ACA CTC GC; R: 5'-ACA TTC CCA GCA GAG TCA CG), MAP3K2 (XM_015290042, F: 5'-TGA GGG ACC CAC CAG AAA GA; R: 5'-GCG AGT CAC GTT CTC TGT CA), AQP4 (NM_001004765, F: 5'-GTG CCA GTA TGA ACC CTG CT; R: 5'-AAG AGC ACC AGC AAG GAC TG), and IGF2BP3 (NM_001006359, F: 5'-GCC TTG GCA GTT GGA GCT AT; R: 5'-AGC TTG GCA TCT GGT CCT TC) were designed using the Primer3 program (National Center for Biotechnology Information [NCBI] database) and synthesized by Genotech Co.
The miRNA gga-miR-9-5p targets 260 genes, with the top-ranking/ top-scoring gene being MAP3K3 (target rank 1, score 100).
Real-time qPCR was used to further examine the expression of the four miRNAs (gga-miR-9-5p, gga-miR-20b-5p, gga-miR196-5p, and gga-let-7d) that showed the highest fold-changes in the IML of NE-induced chickens, compared to their respective controls, as well as the expression of their top-ranking target genes (MAP3K3, MAP3K2, AQP4, and IGF2BP3, respectively) (Figure 2).
This hypothesis is somewhat supported in the case of gga-miR-9-5p, gga-miR-20b-5p, gga-miR-196-5p, and gga-let-7d and the expression of their target genes MAP3K3, MAP3K2, AQP4, and IGF2BP3, respectively in the IML of NE-induced M5.1 chickens.
Upregulated mRNAs Downregulated mRNAs Gene symbol Log2FC Gene symbol Log2FC EPB41L2 8.87 SIPA1L2 -7.60 SH3BP2 7.55 KMT2E -5.52 atm 7.42 PAAF1 -5.69 BTBD18 6.74 ARMC10 -5.07 PRRC2C 6.65 RTN4 -4.96 LPP 6.21 MAP3K3 -4.83 CEP192 6.20 MEF2A -4.81 ncstn 6.00 DIP2A -4.77 PDZD2 5.49 SLC5A3 -4.74 PRKCSH 5.44 POC5 -4.68
The predicted targets of miR-145 were oncogenes myc myelocytomatosis viral-related oncogene (MYCN), FOS, and yamaguchi sarcoma oncogene (YES); cell-cycle promoters such as cyclins D2 and L1; and mitogen-activated protein kinase (MAPK) transduction proteins such as MAP3K3 and MAP4K4 [40].
Those biomarkers included phosphatase and tensin homolog (PTEN), myristolyated alanine-rich protein kinase C substrate (MARCKS) and mitogen-activated protein kinase kinase kinase 3 (MAP3K3).