MALDI-TOF MS

Matrix Assisted Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry. A high-throughput protein sequencing method based on embedding samples in a matrix from which they are desorbed by laser light.

Principle
A laser passes through the substances to be analysed, and causes them to vaporise and their molecules to fly upward into a tube. The time of flight through the tube correlates directly to mass—lighter molecules have a shorter time of flight than heavier ones. MALDI-TOF MS is a potential for measuring the mass of DNA fragments and predicting DNA sequences based on molecular mass.

References in periodicals archive ?

Evaluation of the Biotyper MALDI-TOF MS system for identification of Staphylococcus species.

Accurate identification of atypical Staphylococcus chromogenes plasma-clotting strains causing bovine mastitis/Identificacao acurada de cepas atipicas de Staphylococcus chromogenes que coagulam o plasma envolvidas com mastite bovina

Bu bildiride, kan kulturunde uretilmis Brucella melitensis'in, VITEK[R] MS (bioMerieux, Marcy l'Etoile, Fransa) matriksle desteklenmis lazer dezorpsiyon iyonizasyon ucus zamani kutle spektrometrisi (MALDI-TOF MS) mikrobiyal tanimlama sistemi tarafindan Roseomonas gilardii olarak yanlis tanimlanmasina dikkat cekilmesi amaclanmistir.

Brucella melitensis Misidentified as Roseomonas gilardii by MALDI-TOF MS: Experience of a Clinical Microbiology Laboratory /MALDI-TOF MS'le Roseomonas gilardii Olarak Tanimlanan Brucella melitensis: Bir Klinik Mikrobiyoloji Laboratuvarinin Deneyimi

sakazakii isolates previously identified by conventional biochemical testing, local MALDI-TOF MS analysis (Bruker Biotyper and VITEK MS), locally performed Cronobacter genusand species-specific PCRs, or 16S rRNA gene sequence analysis.

Multicenter Study of Cronobacter sakazakii Infections in Humans, Europe, 2017

In recent times, numerous studies assessed the application of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for the detection of various microorganisms in the diagnostic laboratories (10,11,12,13,14,15,16).

Mass Spectrometry Technology and qPCR for Detection of Enterococcus faecalis in Diabetic Foot Patients

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) can be 10-100 times more sensitive than MBA (1), but is not yet widely available.

Intestinal Colonization and Possible Iatrogenic Botulism in Mouse Bioassay-Negative Serum Specimens--Los Angeles County, California, November 2017

Compared to other analysis, MALDI-TOF MS of peptides begets principally single-charged ions (Mann and Jensen, 2003; Karas and Kruger, 2003).

Identification and Isolation of the Specific Allergens from Mutton by Western Blot, Chromatography and MALDI-TOF/TOF-MS

In this regard, MS (mass spectrometry), particularly MALDI-TOF MS (matrix-assisted laser desorption ionization-time of flight mass spectrometry), has been suggested as an effective alternative for microbial characterization thanks to the high efficiency of proteome profiling, allowing rapid and accurate ID of pathogenic microorganisms, including yeasts and filamentous fungi of medical interest.

Phenotypic typing and epidemiological survey of antifungal resistance of Candida species detected in clinical samples of Italian patients in a 17 months' period

Effect of matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) alone versus MALDI-TOF MS combined with real-time antimicrobial stewardship interventions on time to optimal antimicrobial therapy in patients with positive blood cultures.

Tailoring antimicrobials in febrile neutropenia: using faster diagnostic and communication tools to improve treatment in the era of extensively resistant pathogens

The microbial species identification was carried out using matrix-assisted laser desorption/ionization (MALDI-TOF MS) as described elsewhere [9].

Trueperella pyogenes and Brucella abortus Coinfection in a Dog and a Cat on a Dairy Farm in Egypt with Recurrent Cases of Mastitis and Abortion

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a relatively new technology for the identification of pathogenic organisms.

Clinical Utility of Rapid Pathogen Identification for Detecting the Causative Organisms in Sepsis: A Single-Center Study in Korea

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