MALDI-TOF MSMatrix Assisted Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry. A high-throughput protein sequencing method based on embedding samples in a matrix from which they are desorbed by laser light.
A laser passes through the substances to be analysed, and causes them to vaporise and their molecules to fly upward into a tube. The time of flight through the tube correlates directly to mass—lighter molecules have a shorter time of flight than heavier ones. MALDI-TOF MS is a potential for measuring the mass of DNA fragments and predicting DNA sequences based on molecular mass.