Evaluation of the Biotyper
MALDI-TOF MS system for identification of Staphylococcus species.
Bu bildiride, kan kulturunde uretilmis Brucella melitensis'in, VITEK[R] MS (bioMerieux, Marcy l'Etoile, Fransa) matriksle desteklenmis lazer dezorpsiyon iyonizasyon ucus zamani kutle spektrometrisi (
MALDI-TOF MS) mikrobiyal tanimlama sistemi tarafindan Roseomonas gilardii olarak yanlis tanimlanmasina dikkat cekilmesi amaclanmistir.
sakazakii isolates previously identified by conventional biochemical testing, local
MALDI-TOF MS analysis (Bruker Biotyper and VITEK MS), locally performed Cronobacter genusand species-specific PCRs, or 16S rRNA gene sequence analysis.
In recent times, numerous studies assessed the application of matrix-assisted laser desorption ionization-time of flight mass spectrometry (
MALDI-TOF MS) for the detection of various microorganisms in the diagnostic laboratories (10,11,12,13,14,15,16).
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (
MALDI-TOF MS) can be 10-100 times more sensitive than MBA (1), but is not yet widely available.
Compared to other analysis,
MALDI-TOF MS of peptides begets principally single-charged ions (Mann and Jensen, 2003; Karas and Kruger, 2003).
In this regard, MS (mass spectrometry), particularly
MALDI-TOF MS (matrix-assisted laser desorption ionization-time of flight mass spectrometry), has been suggested as an effective alternative for microbial characterization thanks to the high efficiency of proteome profiling, allowing rapid and accurate ID of pathogenic microorganisms, including yeasts and filamentous fungi of medical interest.
Effect of matrix assisted laser desorption ionization-time of flight mass spectrometry (
MALDI-TOF MS) alone versus
MALDI-TOF MS combined with real-time antimicrobial stewardship interventions on time to optimal antimicrobial therapy in patients with positive blood cultures.
The microbial species identification was carried out using matrix-assisted laser desorption/ionization (
MALDI-TOF MS) as described elsewhere [9].
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (
MALDI-TOF MS) is a relatively new technology for the identification of pathogenic organisms.