MALDI-TOF MS

MALDI-TOF MS

Matrix Assisted Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry. A high-throughput protein sequencing method based on embedding samples in a matrix from which they are desorbed by laser light.

Principle
A laser passes through the substances to be analysed, and causes them to vaporise and their molecules to fly upward into a tube. The time of flight through the tube correlates directly to mass—lighter molecules have a shorter time of flight than heavier ones. MALDI-TOF MS is a potential for measuring the mass of DNA fragments and predicting DNA sequences based on molecular mass.
References in periodicals archive ?
Prior information notice without call for competition: maldi-tof ms instrument for akershus university hospital trust
Bu bildiride, kan kulturunde uretilmis Brucella melitensis'in, VITEK[R] MS (bioMerieux, Marcy l'Etoile, Fransa) matriksle desteklenmis lazer dezorpsiyon iyonizasyon ucus zamani kutle spektrometrisi (MALDI-TOF MS) mikrobiyal tanimlama sistemi tarafindan Roseomonas gilardii olarak yanlis tanimlanmasina dikkat cekilmesi amaclanmistir.
sakazakii isolates previously identified by conventional biochemical testing, local MALDI-TOF MS analysis (Bruker Biotyper and VITEK MS), locally performed Cronobacter genusand species-specific PCRs, or 16S rRNA gene sequence analysis.
In recent times, numerous studies assessed the application of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for the detection of various microorganisms in the diagnostic laboratories (10,11,12,13,14,15,16).
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) can be 10-100 times more sensitive than MBA (1), but is not yet widely available.
Compared to other analysis, MALDI-TOF MS of peptides begets principally single-charged ions (Mann and Jensen, 2003; Karas and Kruger, 2003).
MALDI-TOF mass spectrometry (MALDI-TOF MS) provides a quick and precise technique for bacterial identification that has been lately used in clinical laboratories around the globe [25].
In this regard, MS (mass spectrometry), particularly MALDI-TOF MS (matrix-assisted laser desorption ionization-time of flight mass spectrometry), has been suggested as an effective alternative for microbial characterization thanks to the high efficiency of proteome profiling, allowing rapid and accurate ID of pathogenic microorganisms, including yeasts and filamentous fungi of medical interest.
Effect of matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) alone versus MALDI-TOF MS combined with real-time antimicrobial stewardship interventions on time to optimal antimicrobial therapy in patients with positive blood cultures.
The microbial species identification was carried out using matrix-assisted laser desorption/ionization (MALDI-TOF MS) as described elsewhere [9].
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a relatively new technology for the identification of pathogenic organisms.