Myelin loss was highlighted by luxol fast blue
staining (Figure 2).
Necropsy of two affected siblings revealed multiple sites of hemorrhage, cytoplasmic periodic acid-Schiff and Luxol fast blue
positive inclusions in neurons, and aggregates of foamy macrophages in visceral organs.
Meanwhile, pathological changes in brain were determined using both hematoxylin-eosin and luxol fast blue staining.
The mice brain tissue was embedded in paraffin, and 5-[micro]m-thick sections that had been deparaffinized and rehydrated were stained with hematoxylin and eosin (H & E) or luxol fast blue (LFB).
To stain for myelin content, tissue sections from mice were treated with Luxol fast blue (LFB, Sigma, USA).
Luxol fast blue histologic stain was used to assess the extent of remyelination in corpus callosum after implantation of progesterone pellet.
Although a distinctive feature of spironolactone bodies is their concentric and lamellated pattern, Luxol fast blue
stain can also be used to distinguish these inclusions from other mimickers because of their rich content of phospholipids (Figure 1, F).
The sections were stained with hematoxylin-eosin and cresyl violet with luxol fast blue
and subjected to routine neuropathological examination.
A luxol fast blue
stain to access demyelination yielded negative results, and negative in situ hybridization findings were noted for Epstein-Barr virus-encoded RNA.
Outras 12 amostras (seis animais) foram fixadas em formol tamponado a 10%, incluidas em parafina, clivadas com tres micrometros de espessura e coradas com luxol fast blue
e hematoxilinaeosina (HE).
In the left occipital cortex, Luxol fast blue
staining showed many neurons with Luxol fast blue
positivity in cytoplasmic granular material.
Luxol fast blue
stain showed no evidence of demyelination (Figures 1, A, and 2, A).