Other stains include Luxol fast blue
, cresyl violet, thionin and acid-Schiff.
Myelin loss was highlighted by luxol fast blue
staining (Figure 2).
Meanwhile, pathological changes in brain were determined using both hematoxylin-eosin and luxol fast blue
On frozen sections, Gb3 can be demonstrated with several approaches, including staining with periodic acid-Schiff, Hale, Luxol fast blue
, Oil red O, and Sudan black.
To stain for myelin content, tissue sections from mice were treated with Luxol fast blue
(LFB, Sigma, USA).
Selected panels of serial sections (4-5 [micro]m) were processed for routine hematoxylin and eosin (H&E) staining, Luxol fast blue
staining, and immunohistochemical labeling with anti-Foxp3 and analyzed with a Nikon microscope.
The sections were stained with hematoxylin-eosin and cresyl violet with luxol fast blue
and subjected to routine neuropathological examination.
Necropsy of two affected siblings revealed multiple sites of hemorrhage, cytoplasmic periodic acid-Schiff and Luxol fast blue
positive inclusions in neurons, and aggregates of foamy macrophages in visceral organs.
Parts of the samples were fixed in 4% formaldehyde, paraffin-embedded, 8-[micro]m sections, and stained with hematoxylin and eosin, congo red, as well as luxol fast blue
(not for skin tissues).
Luxol fast blue
stain can be used to distinguish these inclusions from other mimics because of their rich content of phospholipids (F) (hematoxylin-eosin, original magnifications X1.25 [C], X10 [D], and X20 [E]; Luxol fast blue
, original magnification X20 [F]).
The myelinated area was assessed by luxol fast blue
Outras 12 amostras (seis animais) foram fixadas em formol tamponado a 10%, incluidas em parafina, clivadas com tres micrometros de espessura e coradas com luxol fast blue
e hematoxilinaeosina (HE).