100 [micro]L of cell lysate was aliquoted for protein quantification by Lowry protein assay [17] and 900 [micro]L of the remaining cell lysate was mixed and incubated with 1.35 mL ice-cold absolute ethanol for 10 min.
50 [micro]L of each sample was aliquoted for Lowry protein assay. 200 [micro]L of sample was added to scintillation vials with 4 mL of scintillation fluid, vortexed, and counted for radioactivity.
100 [micro]L of cell lysate was aliquoted for Lowry protein assay. 200 [micro]L of the remaining lysate was mixed with 50 [micro]L 0.4 N perchloric acid, gently blown with nitrogen gas, and centrifuged at 13,000 RPM for 60 min at 4[degrees]C.
It is well documented that even low concentrations of urea interfere with the Lowry protein assay [5, 6].
Their main concerns are clustered around the following four issues: (a) possible matrix effect while measuring cTnT in tissue extracts with an ELISA optimized for serum measurement; (b) the influence of 8 mol/L urea on the cTnT ELISA and on the Lowry protein assay; (c) apparent inconsistencies in our data; and (d) what the cTnT immunoassay results were in the human heart extracts.
