Lowry protein assay


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Low·ry pro·tein as·say

(low'rē),
a method for determining protein concentrations using the Folin-Ciocalteu reagent.
Synonym(s): Lowry-Folin assay
[Oliver H. Lowry]

Lowry,

Oliver H., U.S. biochemist, 1910–.
Lowry-Folin assay - Synonym(s): Lowry protein assay
Lowry protein assay - a method for determining protein concentrations using the Folin-Ciocalteu reagent. Synonym(s): Lowry-Folin assay
References in periodicals archive ?
100 [micro]L of cell lysate was aliquoted for protein quantification by Lowry protein assay [17] and 900 [micro]L of the remaining cell lysate was mixed and incubated with 1.35 mL ice-cold absolute ethanol for 10 min.
50 [micro]L of each sample was aliquoted for Lowry protein assay. 200 [micro]L of sample was added to scintillation vials with 4 mL of scintillation fluid, vortexed, and counted for radioactivity.
100 [micro]L of cell lysate was aliquoted for Lowry protein assay. 200 [micro]L of the remaining lysate was mixed with 50 [micro]L 0.4 N perchloric acid, gently blown with nitrogen gas, and centrifuged at 13,000 RPM for 60 min at 4[degrees]C.
We later changed to the more appropriate Lowry protein assay, which led to a downward shift in patient values.
It is well documented that even low concentrations of urea interfere with the Lowry protein assay [5, 6].
Their main concerns are clustered around the following four issues: (a) possible matrix effect while measuring cTnT in tissue extracts with an ELISA optimized for serum measurement; (b) the influence of 8 mol/L urea on the cTnT ELISA and on the Lowry protein assay; (c) apparent inconsistencies in our data; and (d) what the cTnT immunoassay results were in the human heart extracts.