The homogenate was then extracted twice using similar method above and protein concentration in the supernatant was measured by Lowry method
(Ohnishi and Barra, 1978).
The amount of free insulin in the supernatant was measured using the Lowry method
at 756 nm.
Estimation of laccase was performed using Lowry method
9 to determine the concentration difference of laccase obtained from Trametes versicolor and Bacillus subtilis 1133.
In the preparations obtained and eluates of fractions the content of proteins was controlled by Lowry method
(Lowry, Rosenbrough, Farr, Randai, 1951) after dialysis at all stages.
Protein content estimation of each conjugate was done by Lowry method
The protein content of the aqueous extract of seeds (AS) and its fractions (F25, F50 and F75) was determined by the Lowry method
, using bovine serum albumin (BSA) as reference.
P-1 shows 1.24 mg/100 g plants but computer software computed the colour protein by Lowry method
and 1.68 mg/100 g by intensities of stained plant sections and the colour Bradford method respectively.
Part 1: Fundamental Techniques; Part 2: Copper Binding Methods: The Alkaline Copper Reagent: Biuret Assay; The Lowry Method
; The Bicinchoninic Acid Protein Assay; Part 3: Dye Binding Methods: The Udy Method; The Bradford Method - Principles; Bradford Assay - Applications; Part 4: Immunological Methods for Protein Speciation: Immunological Assay: General Principles and the Agar Diffusion Assay; Speciation of Meat Proteins by Enzyme- Linked Immunosorbent Assay; Speciation of Soya Protein by Enzyme-Linked Immunoassay; Determination of Trace Protein Allergens in Foods; Part 5: Protein Nutrient Value: Biological and Chemical Tests for Protein Nutrient Value; Effect of Processing on Protein Nutrient Value; Protein Digestibility - Corrected Amino Acid Scores.
Stock solutions contained 6.39 g/L S-100A1, 1.3 g/L S-100B (as determined using the Lowry method
), and 1 g/L S-100A1-A1, S-100A1-B, and S-100B-B (as specified by the manufacturer).
The Lowry method
is complicated to perform and has low sensitivity.
Protein estimation of chickpea seedling by Lowry method
Total protein content of plasma sample was measured by modified Lowry method
(Lowry et al., 1951) or by determining absorbance of protein solution at 280 nm (based on the fact that 1 mg per ml sample of albumin gives an absorbance of 1.0 at 280 nm).