Isolating the organisms on culture only detects live organism and the identification of isolates may take up to 6-8 weeks using Lowenstein-Jensen medium
. Molecular-based techniques are often superior to conventional techniques in the diagnosis of tuberculosis.
All enrolled index case-patients and household contacts evaluated for active TB were assessed by sputum smear microscopy with Ziehl-Neelsen staining and culture on solid Lowenstein-Jensen medium
. Initial DST was performed by using the proportion method on Lowenstein-Jensen medium
; second-line DST was performed by using the proportion method on Middlebrook 7H11 agar.
scrofulaceum cultures were used to produce sensitins, autoclaved at 120AdegC for 60 minutes, after a 3-time adaptation in nutrient media (Lowenstein-Jensen medium
, Pavlovsky's potato-glycerol medium, Sauton's medium).
Comparison of the Automated Mycobacteria Growth Indicator Tube System (BACTEC 960/MGIT) with Lowenstein-Jensen medium
for recovery of mycobacteria from clinical specimens.
The material needs to be processed for bacilloscopy, culture in Lowenstein-Jensen medium
, and BACTEC technique.
Luh, "Comparison of the BACTEC MGIT 960 with Lowenstein-Jensen medium
for recovery of mycobacteria from clinical specimens," International Journal of Tuberculosis and Lung Disease, vol.
tuberculosis isolates to first-line drugs (INH, streptomycin, RIF, and ethambutol) was performed on Lowenstein-Jensen medium
by proportional method .
This was incorporated into Lowenstein-Jensen medium
at a dilution ratio of 1:20 to obtain a final concentration of 5 [micro]g/mL.
They compared the results of the assay to those based on the absolute drug concentration method in Lowenstein-Jensen medium
. The sensitivity and specificity of the PCR/ESI-MS assay was 100% and 95% for INH resistance, while the sensitivity and specificity was 92% and 97% for RIF resistance, respectively.
Materials and Methods: Antituberculosis drug susceptibility testing of 50 MDR-TB isolates particularly for SLDs, namely p-aminosalicylic acid, ciprofloxacin, amikacin, d-cycloserine, kanamycin, and ethionamide, was conducted on solid Lowenstein-Jensen medium
using the 1% proportion sensitivity method.
The sputa of sputum smear negative cases were inoculated on Lowenstein-Jensen medium
culture medium to isolate the organism and correlate the culture positivity and negativity in smear negative cases.