The histopathologic lesions observed in these goats were indistinguishable from those caused by louping ill virus (10).
JQ646028) was 94% identical to the strain from Britain (EU074000) and 93% identical to the strains from Spain (EU074016) and the Negishi virus (EU074002), thus confirming identity of the Asturian strain louping ill virus. Phylogenetic analysis, conducted by using the unweighted pair-group method analysis tree (Figure 2) shows how the new virus strain is related to the strains from Britain and Spain.
Histopathologic lesions together with molecular genetic results enabled a definitive diagnosis: tick-borne encephalitis caused by an Asturian strain of louping ill virus. These cases confirm the infection in species other than sheep in Spain and the presence of the virus in areas of northern Spain other than the Basque region.
Tracing the origins of louping ill virus by molecular phylogenetic analysis.
Transmission of louping ill virus between infected and uninfected ticks co-feeding on mountain hares.
SINV, Sindbis virus; TBEV, tickborne encephalitis virus; INKV, Inkoo virus; BATV, Batai virus; TAHV, Tahyna virus; UUKV, Uukuniemi virus, WNV, West Nile virus; SFV, Semliki Forest virus; CHIKV, Chikungunya virus; USUV, Usutu virus; LEDV, Lednice virus; SSEV, Spanish sheep encephalomyelitis virus; SFSV, sandfly fever Sicilian virus; GGEV, Greek goat encephalomyelitis virus; CCHFV, Crimean-Congo hemorrhagic fever virus; LIV, Louping ill virus. ([dagger]) Positive reactions to dengue virus or yellow fever virus are not included as they are assumed to represent cross-reactions with WNV or other Japanese encephalitis complex flaviviruses.