lipofection


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lip·o·fec·tion

(lip'o-fek'shŭn),
The process of injecting a lipid-complexed or contained DNA into eucaryotic cells.
[lipo- + transfection]
References in periodicals archive ?
Lipofection is one of the chemical based transient protein expression method which uses liposomes.
These consequences led the researchers to look beyond viruses to deliver the molecules and some of them include non-viral methods like the injection of naked DNA, Lipofection, which can eliminate the potential adverse effects that might be caused due to viral vectors.
[ClickPress, Mon Oct 01 2018] According to the new market research report "Transfection Reagents and Equipment Market by Method (Biochemical (Calcium Phosphate, Lipofection, Dendrimers), Physical (Electroporation, Nucleofection), Viral)), Application (Biomedical, Protein Production), End User - Global Forecast to 2021" , analyzes and studies the major market drivers, restraints/challenges, and opportunities.
[USPRwire, Mon Oct 01 2018] According to the new market research report "Transfection Reagents and Equipment Market by Method (Biochemical (Calcium Phosphate, Lipofection, Dendrimers), Physical (Electroporation, Nucleofection), Viral)), Application (Biomedical, Protein Production), End User - Global Forecast to 2021" , analyzes and studies the major market drivers, restraints/challenges, and opportunities.
In the meantime, lipofection protocols (Liu et al., 2009) and new electroporation technologies including nucleofection (Siemen et al., 2005) have been developed that enable efficient plasmid transfection (Templin et al., 2012) and generation of hPSC lines with more stable transgene expression (Schwanke et al., 2014).
coli were introduced into pig fetal fibroblasts by lipofection, as described below.
In cultures, CRISPR cargo is commonly delivered by lipofection, electroporation, nucleofection, and microinjection.
Wall et al., "Cell tagging with clinically approved iron oxides: feasibility and effect of lipofection, particle size, and surface coating on labeling efficiency," Radiology, vol.
More recently, commercial lipofection reagent Lipofectamine 3000[R] was used to transfect primary NK cells with tumor suppressor microRNA miR-27a-5p inhibitor in a study by Regis et al.
In addition to endogenous substances, some exogenous biomaterials can be loaded into EXO: in vivo drug loading: the genes that encode for the RNA/protein of interest are introduced into exosome-secreting cells by standard gene transfection methods; in vitro drug loading: the drugs of interest are loaded into purified exosomes using standard membrane breaching techniques such as electroporation and lipofection [61].
Within 2-3 days upon transfection (lipofection) with roGFP1 expressing plasmids (see [57]), cultured astrocytes sufficiently expressed roGFP1.
HSA-PEI/pEGFP had been shown to provide better pEGFP transfection efficiency than PEI/pEGFP, jetPEI/pEGFP or lipofection. In order to evaluate whether the effect of HSA-PEI was related to the albumin receptor or not, A549 cells were treated with HSA, ranging from 1 to 50 mg/mL.