double-reciprocal plot

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dou·ble-·re·cip·ro·cal plot

a graphic representation of enzyme kinetic data in which 1/v (on the vertical axis), where v is the initial velocity, is plotted as a function of the reciprocal of the substrate concentration (1/[S]).

Lineweaver,

Hans, U.S. physical chemist, 1907–.
Lineweaver-Burk equation - rearrangement of the Michaelis-Menten equation.
Lineweaver-Burk plot - graphical representation of enzyme kinetic data. Synonym(s): double-reciprocal plot; Woolf-Lineweaver-Burk plot
References in periodicals archive ?
Competitive, noncompetitive and uncompetitive inhibition can be easily distinguished with the use of double reciprocal plot of the Lineweaver-Burk plot.
max] of recombinant EG1 was calculated: In Lineweaver-Burk plot ([R.
5, 5 and 10 [micro]M for cryptotanshinone, and 0, 5, 10 and 20 [micro]M for salvianolic C) were used for the construction of Lineweaver-Burk plot and estimation of [K.
4 mg/ml starch, respectively, as determined by Lineweaver-Burk plot (Figure 2A).
m]s) were estimated from six experiments using the Lineweaver-Burk plot.
The Lineweaver-Burk plot intersected above the x-axis [18,19,20], indicating that HTOR inhibited the reaction in a noncompetitive manner over the substrate range from 0.
To determine the kinetics parameters, Dixon plot was used to determine inhibition constant (Ki) [15] and Lineweaver-Burk plot [16] over the substrate was used at different concentrations 2.
The data were fitted to Michaelis-Menten model and further analyzed using Lineweaver-Burk plot and Dixon plot.
This was indicated by the comparable Michaelis constant (Km) values obtained from the Lineweaver-Burk plot (0.
Km and Vmax values for the enzyme at optimal pH and temperature were determined by Lineweaver-Burk plot [27].