double-reciprocal plot

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dou·ble-·re·cip·ro·cal plot

a graphic representation of enzyme kinetic data in which 1/v (on the vertical axis), where v is the initial velocity, is plotted as a function of the reciprocal of the substrate concentration (1/[S]).


Hans, U.S. physical chemist, 1907–.
Lineweaver-Burk equation - rearrangement of the Michaelis-Menten equation.
Lineweaver-Burk plot - graphical representation of enzyme kinetic data. Synonym(s): double-reciprocal plot; Woolf-Lineweaver-Burk plot
References in periodicals archive ?
5, 5 and 10 [micro]M for cryptotanshinone, and 0, 5, 10 and 20 [micro]M for salvianolic C) were used for the construction of Lineweaver-Burk plot and estimation of [K.
4 mg/ml starch, respectively, as determined by Lineweaver-Burk plot (Figure 2A).
m]s) were estimated from six experiments using the Lineweaver-Burk plot.
The data were fitted to Michaelis-Menten model and further analyzed using Lineweaver-Burk plot and Dixon plot.
This was indicated by the comparable Michaelis constant (Km) values obtained from the Lineweaver-Burk plot (0.
max]) were derived from Lineweaver-Burk plot while inhibition constant ([K.
These plots included Primary Lineweaver-Burk Plot (obtained by reciprocal of reaction velocities versus reciprocal of substrate concentrations), Dixon plot (obtained by reciprocal of reaction velocities versus inhibitor concentrations), Secondary Lineweaver-Burk plot for [K.
Ki value was calculated by Secondary Lineweaver-Burk plot using the slopes of the lines in the Primary Lineweaver-Burk plot versus inhibitor concentrations, while the Secondary Lineweaver-Burk plot of y-intercept in primary plot versus inhibitor concentrations was used to obtain [alpha][K.
A Lineweaver-Burk Plot is a double reciprocal plot in which varying substrate concentrations are plotted against reaction velocities to obtain linear transformation.