Competitive, noncompetitive and uncompetitive inhibition can be easily distinguished with the use of double reciprocal plot of the Lineweaver-Burk plot
max] of recombinant EG1 was calculated: In Lineweaver-Burk plot
5, 5 and 10 [micro]M for cryptotanshinone, and 0, 5, 10 and 20 [micro]M for salvianolic C) were used for the construction of Lineweaver-Burk plot
and estimation of [K.
max] were then calculated using the Lineweaver-Burk plot
4 mg/ml starch, respectively, as determined by Lineweaver-Burk plot
m]s) were estimated from six experiments using the Lineweaver-Burk plot
The Lineweaver-Burk plot
intersected above the x-axis [18,19,20], indicating that HTOR inhibited the reaction in a noncompetitive manner over the substrate range from 0.
To determine the kinetics parameters, Dixon plot was used to determine inhibition constant (Ki)  and Lineweaver-Burk plot
 over the substrate was used at different concentrations 2.
The data were fitted to Michaelis-Menten model and further analyzed using Lineweaver-Burk plot
and Dixon plot.
This was indicated by the comparable Michaelis constant (Km) values obtained from the Lineweaver-Burk plot
As for eupatorin, Lineweaver-Burk plot
illustrated in Fig.
Km and Vmax values for the enzyme at optimal pH and temperature were determined by Lineweaver-Burk plot