Leishman stain


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Related to Leishman stain: Giemsa stain, Wright stain

Leish·man stain

(lēsh'măn),
a polychromed eosin-methylene blue stain used in the examination of blood films for identifying parasites.

Leish·man stain

(lēsh'măn stān)
A polychromed eosin-methylene blue stain used in the examination of blood films.

Leishman stain

A stain containing alkaline methylene blue and eosin in methanol used in genetics to identify chromosomal bands and in blood smear analysis to identify white blood cells and parasitic infections.

Leishman,

Sir William Boog, Scottish surgeon, 1865-1926.
Leishmania - a genus of digenetic, asexual, protozoan flagellates.
Leishman chrome cells - basophilic granular leukocytes (basophils) observed in the circulating blood of some persons with blackwater fever.
Leishman stain - a polychromed eosin-methylene blue stain used in the examination of blood films.
Leishman-Donovan body - the intracytoplasmic, nonflagellated leishmanial form of certain intracellular parasites. Synonym(s): amastigote; L-D body
leishmaniasis - tropical disease that is spread by sandflies.
References in periodicals archive ?
Leishman stain: Air dried FNAC smears were kept on the staining tray, and Leishman stain was poured in a manner similar for blood smear staining.
Efficacy of Leishman stain in cytology--A 30 year's experience.
Leishman stained smears revealed sheets, clusters and singly scattered pleomorphic tumour cells with moderate amount of basophilic cytoplasm, round to oval hyperchromatic nucleus and few showing prominent nucleoli; along with bi-nucleated forms of tumour cells in a background of numerous lymphocytes and haemorrhage.
Photomicrograph of Acute Lymphoblastic Lymphoma: Marrow Aspirate shows Hypercellular Marrow replaced by Lymphoblasts (Leishman Stain 400x).
Photomicrograph of Hypoplastic Marrow Aspirate showing Hypocellularity with Increased Fat Spaces in a 20 years old male (Leishman Stain x100).
The thick smear was stained with Leishman stain. Thin smears were stained by all the three staining methods, Leishman stain, JSB stain and Field's Stain as per standard staining techniques.
In the present study the results obtained by thin smear examination by Leishman stain, JSB stain and Fields stain were same.
The samples were analysed for blast percentage and morphology on leishman stained peripheral blood and bone marrow smears followed by cytochemicalstains (Sudan Black B).
Leishman stained smears from peripheral blood and bone marrow aspirates were examined under microscope for morphology and percentage of blasts.11
Leishman stained bone marrow in our patient showed plasmacytosis and extensive deposition of eosinophilic amorphous PAS positive and Congo Red negative "amyloid-like" material.
Blood samples of all suspected cases of malaria reported in the hospital were drawn for diagnosis and species of parasite was determined by Leishman stained blood smears following WHO recommendations8.
73 cases were selected from in and out patient departments of Gandhi Medical College and associated Hamidia Hospital Bhopal having provisional diagnosis of acute leukemia and leucoproliferative disorder so on basis of leishman stained smears-Peripheral/bone marrow.