Leishman stain

(redirected from Leishman's stain)

Leish·man stain

(lēsh'măn),
a polychromed eosin-methylene blue stain used in the examination of blood films for identifying parasites.
Farlex Partner Medical Dictionary © Farlex 2012

Leish·man stain

(lēsh'măn stān)
A polychromed eosin-methylene blue stain used in the examination of blood films.
Medical Dictionary for the Health Professions and Nursing © Farlex 2012

Leishman stain

A stain containing alkaline methylene blue and eosin in methanol used in genetics to identify chromosomal bands and in blood smear analysis to identify white blood cells and parasitic infections.
Medical Dictionary, © 2009 Farlex and Partners

Leishman,

Sir William Boog, Scottish surgeon, 1865-1926.
Leishmania - a genus of digenetic, asexual, protozoan flagellates.
Leishman chrome cells - basophilic granular leukocytes (basophils) observed in the circulating blood of some persons with blackwater fever.
Leishman stain - a polychromed eosin-methylene blue stain used in the examination of blood films.
Leishman-Donovan body - the intracytoplasmic, nonflagellated leishmanial form of certain intracellular parasites. Synonym(s): amastigote; L-D body
leishmaniasis - tropical disease that is spread by sandflies.
Medical Eponyms © Farlex 2012
References in periodicals archive ?
Blood and bone marrow aspiration slides were stained with Leishman's stain to study red cell morphology and aspects of haematopoiesis.
Caption: FIGURE 1: Hashimoto's thyroiditis: (a) Group 1 smears showing follicular cell clusters infiltrated by few lymphocytes (Leishman's stain x100); (b) Group 2 smears showing follicular cell clusters infiltrated by moderate amount of lymphocytes (Leishman's stain x400); (c) Hurthle cells and increased background lymphocytes (Leishman's stain x400); (d) dense lymphocytes in follicular cells clusters (Haematoxylin & Eosin x100).
The impression smears from heart were immediately fixed in methanol and stained with Leishman's stain as per the standard protocol and examined under microscope.
FNAB samples were routinely stained by Leishman's stain. The incisional or excisional biopsies were fixed in 10% neutral buffered formalin and processed by routine paraffin embedding technique.
Bone marrow aspiration was done under aseptic condition .Slides were stained with Leishman's stain and where needed, special stains like Sudan Black and Periodic acid Schiff were used.
The capillary blood was drawn under complete aseptic precautions, smears were prepared immediately and stained using Leishman's stain following standard protocol.
Venous blood samples collected into ethylenediaminetetraacetic acid tubes and thin tongue-shaped smears were made, labeled, and stained with Leishman's stain. Automated hematology analyzer (Sysmex XP-100) was used to determine neutrophil counts.
Leishman's stain showed mostly lymphocytes and occasional neutrophils.
Peripheral smear was stained by Leishman's stain for all the cases and examined in detail for red cell morphology, platelet count and white cell morphology.
Bone marrow aspirate smears are dried and stained with Leishman's stain. The adequacy, cellularity, hematopoiesis of all three cell lines and percentage of plasma cells estimated by a 500 cell differential count.
The wet-fixed smears were stained with Haematoxylin & Eosin (H/E) stain and the air-dried smears were stained with Leishman's stain. Ziehl- Neelsen (ZN) stain was also done for demonstration of Acid-fast bacilli (AFB).