The LCH group is a clonal neoplastic proliferation of Langerhans type cells expressing CD1a, langerin
, and S100 protein.
Immunohistochemical staining for langerin
(red) in normal skin (a) and the erythematous lesions in atopic dermatitis (b), psoriasis vulgaris (c), or three AE (d-f) patients.
With the development of transgenic mouse models expressing fluorescent proteins under the control of promoters of genes expressed specifically in the dendritic cells, such as CD11c  and Langerin
, it has become possible to visualize the dynamic behavior of LCs in vivo.
The results of immunohistochemical (IHC) staining for CD68, CD163, and FACTOR XIII are positive, with CD1a and Langerin
testing being negative.
The characteristic immunophenotype of LCH includes expression of CD1a, S100 protein, and langerin
(CD207) in LCs.
In brief, antigen-retrieval was performed using citrate buffer followed by incubation overnight with primary monoclonal antibodies directed against CD1a (mouse IgG1 clone MTB1, MONX10315, Monosan), Langerin
(mouse IgG2b clone 12D6, NCL-Langerin, Leica) or HLA-DR (mouse IgG1 clone TAL.1B5, Dako).
Langerhans cells stain positive for CD1a, Langerin
(CD207), S-100 protein, and HLA-DR on immunohistochemistry.
expressing cells promote skin immune responses under defined conditions.
Human CD34+ CD11b- cord blood stem cells generate in vitro a a CD34- CD11b+ subset that is enriched in langerin
+ Langerhans dendritic cell precursors.
Other lectin receptors include mannose receptor and langerin
expressed on intradermal dendritic cells and Langerhans cells (DC-SIGN negative), respectively, which may have HIV-binding affinity.
The Langerhans cells produce langerin
, which binds HIV and helps the cells degrade it and transport the virus to local lymph nodes.
CD1a and langerin
: actin as more than Langerhans cell markers.