system perfused with carbon-13-labeled glucose ([U-[sup.13]C]Glc) can potentially probe the downstream metabolic activities further to [sup.18]F-FDG-6-phosphate in the myocardium .
The chest was then opened, and the hearts were rapidly removed and mounted in Langendorff
mode and perfused at a constant flow rate of 8 ml.g [heart.sup.-1] [min.sup.-1] at 36-37[degrees]C with normal Tyrode containing 140 mM NaCl, 5 mM KCl, 1 mM Mg[Cl.sub.2], 10 mM glucose, 5 mM HEPES, and 1.8 mM Ca[Cl.sub.2] and adjusted to pH7.4 with NaOH bubbled with oxygen.
The aorta was cannulated and connected to a Langendorff
perfusion system filled with warmed (37[degrees]C) Krebs-Henseleit's solution.
system for isolated hearts (Radnoti LTD, Ireland) was used for isolated-heart exposure to 3.1 nM leptin concentration as follows: rats were deeply anesthetized by intraperitoneal injection with sodium pentobarbital plus heparin (as an anticoagulant) before the heart was extracted by thoracotomy and quickly cannulated through the aorta in order to be continuously retrograde perfused with a physiologic Krebs-Henseleit buffer, pH 7.39, with or without leptin .
The technique dates back to Langendorff
, who in 1897 introduced a model of retrograde perfusion of the isolated mammalian heart via the aorta, later known as the Langendorff
Kelley escaped in his vehicle, but Willeford flagged down a passerby, another local identified as Johnnie Langendorff
, and chased Kelley down in a truck.
Previous studies in rator human heart isolated with the Langendorff
technique indicated that maternal autoantibodies have a direct effect on the fetal heart conducting system, and induced fetal bradycardia and CAVB within 15 min. Once CAVB occurs, it is irreversible and most of the children need permanent pacemaker implantation.
, SAYING: "I did what I thought I needed to do.
Willeford jumped into the truck of another local, Johnnie Langendorff
, who drove off in pursuit with him.
The man who confronted Kelley had help from another local resident, Johnnie Langendorff
, who told KSAT TV that he was driving past the church as the shooting happened.
It was then that another resident, (http://www.ibtimes.com/who-johnnie-langendorff-texas-man-chased-down-church-shooter-2610953) Johnnie Langendorff
, pulled up to the scene.
For years, researchers have used the Langendorff
method to remove the heart from an animal in order to introduce fluid through the aorta, the body's largest vessel, then into the artery network of a heart.