Antibody against LPAR1 was purchased from Cayman Chemical (Ann Arbor, MI); antibodies for LPAR2 and LPAR3 were from LifeSpan BioScience (Seattle, WA).
PCR primers were designed for human LPAR1, LPAR2, and LPAR3 for regular PCR.
AA treatment was also verified to also target proliferative factors LPAR1, LPAR3
, LPAR4, LPAR5 and LPAR6.
Role of LPAR3
, PKC and EGFR in LPA-induced cell migration in oral squamous carcinoma cells.
The conceptus derived estrogen induces the expression of many uterine endometrial genes, including AKR1B1, fibroblast growth factor 7 (FGF7), interleukin 1 receptor accessory protein (IL1RAP), lysophosphatidic acid receptor 3 (LPAR3
), secreted phosphoprotein 1 (SPP1), signal transducer and activator of transcription 1 (STAT1), and transient receptor potential vanilloid type 6 (TRPV6) [25-27].
Some drug targets are related to MAPK activation, such as MAP2K4, TLR4, MAPK10, MAPK14, NOX4, ADAR2A, CHRNA7, ERBB2, FLT3, HTR2A, and LPAR3
For example, LPAR1 is highly expressed in the nervous system , LPAR2 in immune organs such as the thymus and spleen , and LPAR3 in reproductive organs such as the ovary and uterus [7, 16, 34, 35].
 demonstrated mRNA expression of three LPA receptors, LPAR1, LPAR2, and LPAR3, in the granulosa-lutein cells from women undergoing in vitro fertilization.
Mice without the Lpar3 gene showed reproductive defects such as uneven embryo spacing and delayed implantation, which is associated with decreased prostaglandin (PG) production (Ye et al., 2005).
Among these receptors, LPAR3 seems to be a key receptor for conceptus implantation in pigs because LPAR3 expression sharply increases in the uterine endometrium on d 12 of pregnancy, when conceptus implantation begins, and LPA upregulates PTGS2 expression through LPAR3 (Seo et al., 2008).
Estrogen also regulates expression of many uterine endometrial genes, including fibroblast growth factor 7 (FGF7), lysophosphatidic acid receptor 3 (LPAR3), and secreted phosphoprotein 1 (SPP1) during the implantation period (Jaeger et al., 2001).
Estrogen also affect expression of many uterine endometrial genes such as FGF7 (Ka et al., 2001), SPP1 (White et al., 2005), LPAR3 (Seo et al., 2008), signal transducers and activator of transcription 1 (STAT1) (Joyce et al., 2007), and stanniocalcin 1 (STC1) (Song et al., 2009).