Algunos de ellos son el Forhead Box G1 (FoxGl), LIM Homebox 2 (Lhx2
), Paired Box 6 (Pax6) y Empty Spiracle Homologue 2 (Emx2), ademas de ciertos precursores intermedios de diferenciacion como los factores de transcripcion Tbr2, neurogenina 2 y Cut-like Homebox 1 y 2 (Cux 1 y 2) (13,14).
In a previous study, an exogenous gene (Lhx2
) was overexpressed in OP9 (OP9Lhx2) (35) to enhance hematopoietic differentiation of hPSCs.
Studies on murine hair follicles have also revealed expression of, inter alia, CK19 [8, 22] and numerous transcription factors, that is, Sox9, Lgr5, Gli1, Hopx, LHX2
, Nfatc1, and Tcf3 [8, 25].
Within the neural epithelial-derived optic vesicle, animal studies have demonstrated that Pax6 acts together with additional transcription factors such as Pax2, Six6, Gsx2, Pax5, and Lhx2
to regulate invagination to form the bilayered cup that will become the retina, retinal pigmented epithelium, and iris pigmented epithelium [8, 10, 12, 46].
Molecular cloning of LIM homeodomain transcription factor Lhx2
as a transcription factor of porcine follicle-stimulating hormone beta subunit (FSHb) gene.
Koike et al., "miR-124a is required for hippocampal axogenesis and retinal cone survival through Lhx2
suppression," Nature Neuroscience, vol.
Xiao et al., "miR-1238 inhibits cell proliferation by targeting LHX2
in non-small cell lung cancer," Oncotarget, vol.
Tole and her group found a clue to how neurons form layers in the brain, besides discovering a gene called Lhx2
that's responsible for the formation of the cerebral cortex.
Immunocytochemistry showed that these cells expressed the corresponding markers, such as SOX1, PAX6, OTX2, LHX2
, and SIX3 (Figures 2(d)-2(h)).
To validate microarray data, we analysed SFN, FOXN1, Krt15, LHX2
, CD34, and SOX9 gene expression by using mouse-predesigned oligos (Assay IDs: Mm.PT.58.42262048.g, Mm.PT.58.13135783, Mm.PT.58.5528981, Mm.PT.58.6480133, Mm.PT.58.8626728, and Mm.PT.58.42739087, resp., IDT[R]) and K6b-designed oligos (5'-CATCAAATACACCACCAGCG-3' (forward) and 5'-AAGCAGCCAAAAAGAGAAGC-3' (reverse)).
The vertebrate ttx-3 homolog Lhx2
and the lin-11 homolog Lhx1 are indeed expressed in the diencephalon, which gives rise to the thermoregulatory hypothalamus (9, 10).
When compared with normal group, there were 6 upregulated HOX genes in MSC group (Hhex, Hox a5, Hoxb5, Hoxb7, Hox c9, and Lhx2