In order for LFA-1 to bind to ICAM1, LFA-1 must be activated to a high affinity state, a process which can be imaged via radiolabeled DANBIRT .
3D autoradiography was implemented in the study to allow both functional and advanced anatomical assessment of the presence of LFA-1 expression in cardiovascular tissues.
Functional and morphological assessment of atherosclerotic plaque using [sup.111]In-DANBIRT relies on LFA-1's restricted expression in leukocytes and DANBIRT's low molecular weight and specificity.
DANBIRT is a chemically restructured radioligand capable of binding to LFA-1 expressed by leukocytes (M.W.
Hooftman-den Otter et al., "Expression of the leucocyte integrin LFA-1 (CD11a/CD18) and its ligand ICAM-1 (CD54) in lymphoid malignancies is related to lineage derivation and stage of differentiation but not to tumor grade," Leukemia, vol.
Back, "Stereodivergent synthesis of the LFA-1 antagonist BIRT-377 by porcine liver esterase desymmetrization and Curtius rearrangement," Organic and Biomolecular Chemistry, vol.
Barbas 3rd, "Total synthesis of LFA-1 antagonist BIRT-377 via organocatalytic asymmetric construction of a quaternary stereocenter," Organic Letters, vol.
In contrast, the increase in LFA-1 MFI values above the values recorded in healthy Controls with no known exposition to cases of active TB was observed on monocytes from patients with active TB and their healthy contacts but also on monocytes from patients with nonmycobacterial lung disease.
The decreased frequency of monocytes with mCD14 and LFA-1 coexpression in PBML from TB and NMLD patients could have been caused by intensive recruitment of such cells into lungs.
Schlesinger, "Mycobacterium tuberculosis-infected human macrophages exhibit enhanced cellular adhesion with increased expression of LFA-1 and ICAM-1 and reduced expression and/or function of complement receptors, FcyRII and the mannose receptor," Microbiology, vol.
Behar, "'The LFA-1 adhesion molecule is required for protective immunity during pulmonary Mycobacterium tuberculosis infection" Journal of Immunology, vol.
Figure 10: The percentage of monocytes designated LFA-1 high ([LFA-1.sup.hi]) and LFA-1 low ([LFA-1.sup.10]) in the study groups.