Genetic relationships among the 30 trees were examined on the basis of those isozymes for which more than one allele was detected (LAP1, MDH3, IDH1, IDH3, SKDH1, 6PGDH2 and PGI2).
Locus Allele ELL HYB RUB LAP1 1 0.05 0.05 0.20 2 0.50 0.50 0.65 3 0.20 0.20 0.05 4 0.25 0.25 0.10 MDH3 ls [*] 0.00 0.00 0.05 1 1.00 1.00 0.95 IDH1 1 0.15 0.00 0.00 3 0.30 0.50 0.40 4 0.20 0.00 0.20 5 0.35 0.50 0.40 IDH3 1 0.00 0.10 0.00 2 0.80 0.90 1.00 3 0.20 0.00 0.00 SKDH1 2 0.15 0.20 0.40 3 0.10 0.10 0.25 4 0.05 0.00 0.00 5 0.70 0.70 0.35 6PGDH2 1 0.00 0.00 0.05 2 1.00 1.00 0.95 PGI2 3 1.00 0.55 0.55 5 0.00 0.45 0.45 (*.)A slow allele not previously reported Results of Mantel tests comparing relationships among four distance matrices.
The 13 enzyme systems (and 18 associated, polymorphic loci) assayed for this study were acid phosphatase (Acp), diaphorase (Dia2), fluorescent esterase (Fe2, Fe3), glutamate oxaloacetate transaminase (Got1, Got2), isocitrate dehydrogenase (Idh), leucine aminopeptidase (
Lap1, Lap2), malate dehydrogenase (Mdh), malic enzyme (Me), 6-phosphogluconate de hydrogenase (6Pgd1, 6Pgd2), phosphoglucose isomerase (Pgi2), phosphoglucomutase (Pgm1, Pgm2), shikimate dehydrogenase (Skdh), and triosphosphate isomerase (Tpi1).