limulus amebocyte lysate test

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limulus amebocyte lysate test

(lĭm′ū-lŭs),

LAL test

A test used to detect minute quantities of bacterial endotoxins and to test for pyrogens in various materials; it is also used to detect septicemia due to gram-negative bacteria. Limulus amebocyte lysate is formed from the lysed circulating amebocytes of the horseshoe crab (Limulus polyphemus).
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The LAL test is further sub segmented in to chromogenic tests, turbidimetric tests, and gel clot tests.
The sensitivity, reliability, and ease-of-use of the LAL test has made it the preferred method in most laboratories.
We have, therefore, performed two tests to check for the presence of endotoxins in HM: the LAL test and the Fluorogenic one (Lonza Verviers SPRL) for which we obtained two contradictory results.
The LAL test, based on coagulation of blood from the horseshoe crab (Limulus polyphemus), detects only one class of pyrogen endotoxins from gram-negative bacteria leaving patients at risk from "non-endotoxin" pyrogens such as gram-positive toxins, viruses and fungi.
The LAL test used to detect endotoxins in humans is derived from the blue, copper-based blood of the horseshoe crab.
For example, numerous published studies of body fluids as diverse as bronchoalveolar lavage,[1] cerebrospinal fluid,[2] and urine[3] have found that endotoxin detection with the LAL test can be used as a reliable marker of gram-negative infection.
In the presentation entitled "Rapid ultrasensitive method for endotoxin detection utilizing T2MR and LAL test reagents", the T2Endotoxin assay was compared with two commercially available turbidimetric LAL assays.
The advantage of the MAT is even more pronounced in comparison to the LAL test which only identifies endotoxins and is subject to false positive reactions to glucans [8].
Exclusive supply of material for LAL test in dialysis water (search for bacterial endotoxins in dialysis water).