The reference compounds of L-rhamnose
monohydrate (BCBL0552V, 98%) and xylose (WXBB5741V, 98%) were purchased from Sigma-Aldrich Co., Ltd., and D-mannose (LOTD1429046, 98%) and 3-Methyl-1-phenyl-2-pyrazoline-5-one (PMP, 99%) were from Aladdin Co., Ltd., and glucose (LOT110833-201205,98%) and galactose (LOT100226-201105, 98%) were purchased from the National Institute for Food and Drug control (Beijing, China).
Methyl [alpha]-L-rhamnopyranoside (2): The title compound (2) was prepared from L-rhamnose
(Merck) and anhydrous methanol with Amberlite IR 120 ([H.sup.+]) ion exchange resin in 82% yield as a crystalline solid, mp 109-110 [degrees]C (Lit.
Because of variability in the utilization of L-Rhamnose
and Adonitol, E.
The various monosaccharides and their analogs tested as candidates for an internal standard were found to have the following retention times (in min): D-arabinose, 21.2; D -altrose, 28.4; D-allose, no peak; 2-deoxy-D-glucose, 12.5; L-rhamnose
, 13.9; 3-O-methyl-n-glucose, 20.3; methyl-a-n-glucoside, 7.3; and methyl-[beta]-D-glucoside, 7.4.
Flaxseed gum contains two types of polysaccharide, a neutral arabinoxylan and a pectie-like acidic polysaccharide containing D-galactose, L-rhamnose
and D-galaeturonic acid.
Pectin is a polymer of partially methoxylated alpha, 1,4 linked D-galacturonic acid that is interrupted by dispersed or alternating 1,2 linked L-rhamnose
units that are linked to neutral sugars such as L-galactose and L-arabinose.
The separated sugars were identified by the sign of their optical rotation, which were found as L-rhamnose
([[alpha]]D20 + 8.5 ) in 1 and D-glucose ([[alpha]]D20 -53.5 ) and L-rhamnose
in 2 .
At predetermined intervals, 25-gram samples were withdrawn, blended with 225 ml of diluent, and enumerated onto a culture medium of 1% L-Rhamnose
MacConkey agar (RMAC).
The strain reduced nitrate to nitrite, produced N-acetyl-[beta]-glucosamidase, phosphatase, [alpha]- and [beta]-galactosidases, [alpha]- and [alpha]-glucosidases, and assimilated L-arabinose, L-fucose, D-glucose, maltose, D-mannose, D-melibiose, L-rhamnose
, sucrose, and salicin with Api 20NE, Api ID32GN (bioMerieux), and ID-GNB biochemical galleries.