Effects on cloned Kv4.3
channels and native transient outward current.
Methods: Uterine tissue samples from 22 patients (cases) with histologically conﬁrmed AM and 12 (controls) with cervical intraepithelial neoplasia were collected for both immunohistochemistry and real-time polymerase chain reaction to detect the expression of large conductance calcium- and voltage-sensitive K [sup]+ channel (BKCa)-a/[sz] subunits, voltage-gated potassium channel (Kv) 4.2, and Kv4.3. Student's t-test was used to compare the expression.
[sup] Here, we aimed to measure the expression levels of the BKCa-a and -[sz] subunits, Kv4.2, and Kv4.3 in MSCMs from women with AM to explore the pathogenesis of AM with the aim of developing new therapies to prevent or treat it.
Serial 4-mm sections were immunostained for BKCa-a, BKCa-[sz], Kv4.2, and Kv4.3 using standard techniques.
BKCa-[sz] (1:300 v/v) Kv4.2 (1:300 v/v) or Kv4.3 (1:200 v/v), all diluted in phosphate-buffered saline (PBS).
Detection of BKCa-a, BKCa-[sz], kv4.2, and kv4.3 in uterine tissues with and without adenomyosis
Casis, "Differential modulation of Kv4.2 and Kv4.3 channels by calmodulin-dependent protein kinase II in rat cardiac myocytes," American Journal of Physiology--Heart and Circulatory Physiology, vol.
Reihill et al., "Regulation of Kv4.3 currents by [Ca.sup.2+]/calmodulin-dependent protein kinase II," American Journal of Physiology--Cell Physiology, vol.
Women showed particularly lower atrial expression levels of several important genes encoding for potassium channels, including Kv4.3
, KChIP2, Kv1.5 and Kir3.1.
More recently, it was reported that chronic hypoxia also decreases the mRNA expression of Kv1.1, Kv1.5, Kv2.1, KV4.3
, and Kv9.3 a subunits in cultured rat PASMCs [20, 25].
Remodeling of Kv4.3
potassium channel gene expression under the control of sex hormones.
reported that electrical stimulation of hESC-derived cardiomyocytes at 1 Hz increased expression of cardiac-specific genes (such as SCN5A, MLC2V, and Kv4.3
), and a higher percentage of cardiomyocytes showed longer action potential duration, but electrical stimulation did not affect resting membrane potential or action potential upstroke velocity .