Some slides were also prepared and stained with von Kossa for the detection of the presence of microcalcifications within the tumor using a von Kossa Stain
Kit (IHC World).
The results of histochemical testing of the rosette crystals using von Kossa stain
and Pizzolato's method were consistent with previously reported staining characteristics of calcium oxalate crystals [1, 2].
Special stains including Periodic acid Schiff (PAS) with diastase, Perl's stain, von Kossa stain
were applied which highlighted MG bodies (fig-1b,c,d,e).
Case###Location###Iron Perl's Stain###Copper Rhodanine Stain###Calcium - Von Kossa Stain
Histopathological examination would show a focus of a well-circumscribed, round, basophilic substance, which would stain black with the Von Kossa stain
. It may be located in the upper dermis, surrounded by thick collagen fibers and sometimes by epithelioid and multinucleated giant cells .
While frank calcific changes could not be identified in the breast cores by either hematoxylin and eosin stain or Von Kossa stain
, the histologic pattern of extensive ischemic necrosis was felt compatible with the clinical setting of systemic calciphylaxis involving the substance of the right breast, and the immunohistochemical studies helped to further exclude an entirely necrotic malignancy.
Michaelis-Gutmann bodies stain blue with Haematoxylin-eosin and are positive for calcium and iron with the von Kossa stain
and the Perl's stain, respectively.
The classic histologic picture with pseudoxanthoma elasticum skin lesions is fragmentation and clumping of elastic tissue evident on Verhoeff-van Gieson stain, and calcification on yon Kossa stain
. (7) Patients who have angioid streaks on funduscopic examination but no visible skin lesions have received a diagnosis of pseudoxanthoma elasticum based on the biopsy results of scars or flexural skin of the neck or axillae.
Analysis of von Kossa stain
positive areas showed that hMSC seeded on CGCS, and cultured under differentiation conditions for 14 days, 0.28% (mean, SD= 0.03%, n=3) of the image area was covered by calcium deposits.
Paraffin-embedded liver sections (5 [micro]m) were stained with hematoxylin-eosin (HE) to evidence necrosis and cellular inflammation or with von Kossa stain
to evidence calcifications.
With von kossa stain
, calcium deposits are found in approximately 75% of the tumours.