Klenow fragment


Also found in: Wikipedia.

Kle·now frag·ment

carboxyl terminal fragment of DNA polymerase I; contains polymerase as well as 3' → 5' exonuclease activity to edit out mismatches.

Klenow fragment

treatment of the enzyme DNA polymerase 1 with subtilism produces two cleavage products, a small fragment which is unstable and a large fragment, called the Klenow fragment, which retains both the 5′-3′ polymerase and the 3′ exonuclease activities, but not the 5′ exonuclease activity, of the intact enzyme, together with the pyrophosphorolysis and pyrophosphate exchange activities. Used to synthesize DNA complementary to single-stranded DNA template by adding nucleotides to the free 3′-hydroxyl end of an annealed DNA primer.
References in periodicals archive ?
d]) from 25 to 200 nmol/L for thrombin (26), whereas the Klenow fragment polymerase has a [K.
The other heating block was set at 30[degrees]C, the optimal temperature for the Klenow fragment of E.
5-8] The only essential difference between the two is that TUNEL uses the enzyme terminal deoxyribonucleotidyl transferase,[6] whereas ISEL uses DNA polymerase I or its Klenow fragment.
The ends of the annealed OLs were filled-in by means of the Klenow fragment of DNA polymerase and random primer kit solutions (Life Technologies, Inc.
Protease-mediated allele-specific extension (PrASE) is a flexible assay and can be performed at lower temperatures using thermolabile enzymes, such as the Klenow fragment, or at increased temperatures using thermostable DNA polymerases, such as Taq polymerase.
The fragments were subsequently labeled with biotin, using the exonuclease-free Klenow fragment of Escherichia coli DNA polymerase I to fill in the recessive ends with dGTP, dCTP, dTTP, and biotin-14-dATP.