(8) Type 1 DM (1) The total protein was determined by the
Kjeldahl method, lactose by enzymatic hydrolysis and fat by gravimetry after extraction with methylene chloride by the modified Roese-Gottlieb method Bitman et al.
The ammonia nitrogen (N-NH3) concentration was determined after samples were centrifuged at 3,000 rpm/15 min, using the supernatant for analysis by
Kjeldahl method INCT-CA N-007/1 [14].
The first test was ammonia determination by
Kjeldahl method; six bacterial cultures were kept in soil for two weeks to find the microorganism, which could highly produce ammonia.
The a-amino nitrogen was determined by formol titration according to the method as described by Taylor [10], and the total protein nitrogen was determined by the
Kjeldahl method [11].
roseapbrunner for WSPP and WSPC, respectively, in comparison with that of its protein content, which was determined by the
Kjeldahl method [16].
Nitrogen, Sulfur, pectin, lignin, cellulose and hemicellulose were determined for acid digestion according to
Kjeldahl method, which consists in the destruction of the sample with concentrated sulfuric acid in boiling, thus separating the nitrogen from its bond matrix and transforming into ammoniacal nitrogen, with a heating period of 4.5 h at 400[grados]C (15).
Protein determination: Protein in the sample was determined by
Kjeldahl method (Barbano and Clark, 1990).
The protein content of the samples was analyzed using the analytical
Kjeldahl method. A protein-peptide profile analysis was conducted using sodium-dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and imaging analysis software.
The percentage of carbon was determined by dichromatometry and [N.sub.total] by the
Kjeldahl method. Sodium was determined by dilute hydrochloric acid solution and was subsequently determined using flame spectrophotometric apparatus.
The protein content in the supernatant was determined by the
Kjeldahl method and percent protein solubility was calculated as follows:
The N contents of grains were obtained by the
kjeldahl method [15] and multiplied by 6.25 based on dry weight to calculate grain protein content (%).
Nitrogen content of dried camel milk was determined by
Kjeldahl method and then multiplying that by a factor 6.37.
