(8) Type 1 DM (1) The total protein was determined by the Kjeldahl method
, lactose by enzymatic hydrolysis and fat by gravimetry after extraction with methylene chloride by the modified Roese-Gottlieb method Bitman et al.
The ammonia nitrogen (N-NH3) concentration was determined after samples were centrifuged at 3,000 rpm/15 min, using the supernatant for analysis by Kjeldahl method
INCT-CA N-007/1 .
The first test was ammonia determination by Kjeldahl method
; six bacterial cultures were kept in soil for two weeks to find the microorganism, which could highly produce ammonia.
The a-amino nitrogen was determined by formol titration according to the method as described by Taylor , and the total protein nitrogen was determined by the Kjeldahl method
roseapbrunner for WSPP and WSPC, respectively, in comparison with that of its protein content, which was determined by the Kjeldahl method
Nitrogen, Sulfur, pectin, lignin, cellulose and hemicellulose were determined for acid digestion according to Kjeldahl method
, which consists in the destruction of the sample with concentrated sulfuric acid in boiling, thus separating the nitrogen from its bond matrix and transforming into ammoniacal nitrogen, with a heating period of 4.5 h at 400[grados]C (15).
Protein determination: Protein in the sample was determined by Kjeldahl method
(Barbano and Clark, 1990).
The protein content of the samples was analyzed using the analytical Kjeldahl method
. A protein-peptide profile analysis was conducted using sodium-dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and imaging analysis software.
The percentage of carbon was determined by dichromatometry and [N.sub.total] by the Kjeldahl method
. Sodium was determined by dilute hydrochloric acid solution and was subsequently determined using flame spectrophotometric apparatus.
The protein content in the supernatant was determined by the Kjeldahl method
and percent protein solubility was calculated as follows:
The N contents of grains were obtained by the kjeldahl method
 and multiplied by 6.25 based on dry weight to calculate grain protein content (%).
Nitrogen content of dried camel milk was determined by Kjeldahl method
and then multiplying that by a factor 6.37.