No fungal elements were seen in the Gram stain, and the acid-fast stain using a concentrated Kinyoun stain was negative for acid-fast bacilli.
* No acid-fast bacilli seen (modified Kinyoun stain)
was 100% sensitive and specific for Nocardia spp.
revealed oval asci with 1-4 ascospores inside, a characteristic finding of Saccharomyces genus (Figure 1(d)).
A modified acid-fast Kinyoun stain
was also positive.
The purpose of this study was to detect and confirm the presence of acid fast bacilli in lymph node biopsies using routine Ziehl Neelsen stain, Kinyoun stain
and fluorescence staining and also to determine the best, rapid and more cost effective technique of staining for the detection of acid fast bacilli.
Both patients had previously received a positive diagnosis by FSSM, which was confirmed by SSM with a modified Kinyoun stain
. (9,11) The findings for one patient were reported as 1 + smear positive and for the other, as [3.sub.+] smear positive.
A cytospin slide prepared from the broth was positive for acid-fast bacilli by Kinyoun stain
. The MGIT broth was subcultured to a Middlebrook 7H10/S7H11 agar biplate and incubated at 37[degrees]C in 5% C[O.sub.2] to obtain a pure culture of the organism for subsequent identification.
Decolorized with HCL (95% ethanol, rinsed again with tap water and stained with brilliant green for 30 sec; (Becton Dickinson-Franklyn, Lakes, NJ, TB Kinyoun stain
A Kinyoun stain
from a colony on the Lowenstein-Jensen agar was performed and interpreted as negative (figure 3).
It can be demonstrated in direct smears by modified Kinyoun stain
, in which acid-alcohol decolorizer is substituted with a low-concentration inorganic acid.
All bone marrow specimens were positive for MTBC by culture, or were either positive for AFB by Kinyoun stain
or contained granulomas.