Kinetochore motors drive congression of peripheral polar chromosomes by overcoming random arm-ejection forces.
Spatial regulation of kinetochore microtubule attachments by destabilization at spindle poles in meiosis 1.
A mechanomolecular model for the movement of chromosomes during mitosis driven by minimal kinetochore
When metaphase-arrested oocytes of Chaetopterus were exposed to colchicine, the spindle birefringence gradually disappeared as the fibrils depolymerized (the kinetochore fibers were the longest to persist), but the depolymerizing filaments actually led the chromosomes and inner spindle pole to the cell surface, where the outer meiotic spindle pole was attached.
Maloriented bivalents have metaphase positions at the spindle equator with more kinetochore microtubules to one pole than to the other.
Separase would be active before all kinetochores
were attached to spindle fibers.
For further MN analysis, we stained kinetochores by incubating the fixed cell preparations with CREST serum (Chemicon, Temecula, CA, USA) for 1 hr in a humidified chamber at 37[degrees]C.
Furthermore, staining of kinetochores with anti-kinetochore serum (CREST) allows discrimination of clastogenic effects from aneuploidy.
The scientists also detected presenilins in kinetochores
, which are specialized protein complexes on chromosomes, and in centrosomes, structures found just outside the nuclear membrane.
We've shown that you don't need kinetochores
is a large multi-subunit and multi-copy protein complex that bridges mitotic chromosomes with spindle microtubules.
Our priority will be to characterize mutants defective in mono-orientation of sister kinetochores
during the first meiotic division, as this is the least understood aspect of meiotic chromosome segregation.