In reality megabase deletions cannot be distinguished from kilobase
deletions because of the size limitation of lambda phage to be packaged.
, which is at the small end of the range reported for other insects (Solignac et al.
Abbreviations: BAC, bacterial artificial chromosome; EST, expressed sequence tagged site; kb, kilobase
; kbp, kilobase
pairs; Mb, megabase; STC, sequence tagged connector; YAC, yeast artificial chromosome.
With PCR as the "front end" enabling the amplification of discrete regions that can be covered by an individual Sanger sequencing read -- about one kilobase
-- the bottleneck becomes apparent when used with new sequencing techniques.
The project focuses on the sequencing of a 500 kilobase
region on chromosome 2q13 in 25 human genomes.
SNPs are the most abundant polymorphisms in the human genome with one SNP occurring each kilobase
on average (13).
deletion (CF40 kbdel4-10) removes exons 4 to 10 of the cystic fibrosis transmembrane conductance regulator gene.
Abbreviations: ASAP, arbitrary signatures from amplification profiles; bp, base pairs; cpDNA, chloroplast DNA; DAF, DNA amplification fingerprinting; kbp, kilobase
pairs; Myr, millions of years; PCR, polymerase chain reaction; RAFLP, restriction amplification fragment length polymorphism; RAPD, random amplified polymorphic DNA; RFLP, restriction fragment length polymorphism; SSR, simple sequence repeat.
Abbreviations: cM, centimorgan; kb, DNA kilobase
pairs; PCR, polymerase chain reaction; RAPD, random amplified polymorphic DNA; RFLP, restriction fragment length polymorphism; STS, sequence tagged site.
Abbreviations: AFLP, amplified fragment length polymorphism; kbp, kilobase
pairs; MAS, marker-assisted selection; PCR, polymerase chain reaction; RAPD, random amplified polymorphic DNA; RFLP, restriction fragment length polymorphism.
Mitochondrial DNA (mtDNA) is 16 kilobases
, circular, and encodes 13 protein, 22 tRNA and two rRNA genes.
BAC clones with insert sizes in excess on 100 kb are desired if a library is to be used for assembly of contigs spanning hundreds of kilobases