MIB1

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MIB1

A monoclonal antibody which recognises a formalin-fixation-resistant epitope on the cell proliferation-associated Ki-67 antigen. MIB1 is a reliable marker of cell proliferation, and can be used for image analysis of paraffin-embedded tissues; Ki-67 is a short-lived nonhistone protein expressed during the G1, S and G2/M phases of the cell cycle, but not in the G0 (resting period) phase.

Image analysis of MIB1 may be an alternative method for objectively, reproducibly and reliably classifying dysplastic changes of the cervical epithelium. Its presence is regarded as an independent prognostic indicator in breast cancer—the presence of MIB-1 is associated with a slight increased tumour aggressiveness, but is a poor indicator of prognosis.
References in periodicals archive ?
Mouse anti-human ki-67, Mib-1 clone, Ready for use; Monocl.
Specifically, Enzo's validated p16, a marker used extensively as a key diagnostic and prognostic biomarker of several cancers, is the latest addition to the company's growing immunohistochemistry pipeline, including, among others, Ki-67, Her2 and p53.
Immunohistochemical findings revealed negative for PAS [Figure 1]d, S100, HMB-45, CEA, and GCDFP15 [Figure 1]e, positive for Ki-67 (<30%), CK34[sz]E12 [Figure 1]f, and P63 [Figure 1]g, and weakly positive for CAM5.
In addition, since previous investigators reported that Ki-67 labeling index was extremely low both in primary and metastatic lesions of GP, Ki-67 labeling index may have limited value to evaluate the malignant potential of GP.
Objective: This study aimed to evaluate diagnostic parameters such as mitotic count and tumor size in; leiomyosarcomas (LMSs) and benign uterine smooth muscle tumors (USMTs) and to define the diagnostic value and prognostic significance of the Ki-67 proliferation index and p16, p53, and bcl-2 expressions by immunohistochemical (IHC) methods.
Immunohistochemical analyses included staining with Ki-67, p53, epidermal growth factor receptor (EGFR), cytokeratin (CK) 8, CK14, CK17, and CK18.
In addition, some information about the molecular mechanism underlying the effects of VPA on the male reproductive system, especially effects on spermatogenesis and sex hormone production including testicular Ki-67, phosphorylated proteins, steroidogenic acute regulatory protein (StAR), and cholesterol side-chain cleavage enzyme (P450scc) has been published by our group (Sukhorum et al.
Results determination: If the tumor cell nucleus was dark brown and percentage of positive cells was larger than 10%, Ki-67 was determined as positive; otherwise, it was determined as negative.
Immunohistochemistry was done in our case and showed a cytoplasmic granular positivity with Ki-67, however, there was no membrane positivity (Figure 1e).
Studies have identified four major subtypes of breast cancer according to immunohistochemistry these are, (1) luminal A, (ER+ve and/or PR+ve, Human Epidermal Growth Factor Receptor 2 (HER 2)-ve, Ki-67 14%) OR, HER2+ve with any Ki-67) (3) Her2 over expressed (ER-ve, PR-ve, and HER2+ve) and (4) Triple negative (ER-ve, PR-ve, and HER2-ve)9-12.
Carpinteria, CA) for Ki-67 and p63, or in an ethylenediamine tetra-acetic acid buffer solution at pH 9.
In the present study, GMS-10 and DBTRG-05MG human GBM cell lines were used as a model to examine selenium entering the cell, cell proliferation, cytotoxicity, DNA fragmentation and Ki-67 protein expression in selenomethionine treated and non-treated groups.