Therefore, we examined a possible influence of PRGF on human keratinocyte differentiation by analyzing the influence of PRGF on the expression of keratin 1, keratin 10, transglutaminase-1, and involucrin in primary human keratinocytes as indicators of their differentiation status.
The following intron spanning primers were used: keratin 1: 5-CTT CTT CAG cCc CTC AAT GT-3' (forward primer) and 5-GTA CCT GGT TCT GCT GCT CC-3' (reverse primer); keratin 10: 5-TGA AAA GCA TGG CAACTC AC-3' (forward primer) and 5-TGT CGA TCT GAA GCA GGA TG-3' (reverse primer); involucrin: 5-CTG CCT CAG CCT TAC TGT GA-3' (forward primer) and 5-GGA GGA GGA ACA GTC TTG AGG-3' (reverse primer); and transglutaminase-1: 5-CCC TCA CCA ATG TCG TCT TC-3' and 5-TCA CTG TTT CAT TGC CTC CA-3'.
Analyses of the Influence of Vivostat PRF on the Epidermal Expression of Keratin 1, Keratin 10, Transglutaminase-1, and Involucrin In Vivo.
To investigate a possible influence on the keratin 1, keratin 10, transglutaminase-1, and involucrin gene expression, we stimulated the keratinocytes with different concentrations of PRGF for 24 hours.
These included oxidative defense (PRDX2 was among the 333 always differentially expressed genes (Figure 2(c)); HSP27 and its phosphorylated form were previously found to be central, studying the redox response in THP-1 monocytes ; NQO1 was also found to be redox-controlled using cold plasma ), cellular damage and apoptosis (histone H2AX and its phosphorylation are prominent markers of genomic DNA damage; Puma  and p21  are involved in redox-related apoptosis induction), and cellular structure (keratin 1 showed the strongest regulation among all keratins (Table 2); [beta]-actin served as housekeeping protein).
Dvorakova et al., "Modulation of keratin 1, 10 and involucrin expression as part of the complex response of the human keratinocyte cell line HaCaT to ultraviolet radiation," Interdisciplinary Toxicology, vol.
Puma and p21 ((b)(I), (b)(II)) were upregulated after chronic but not acute oxidative challenge whereas keratin 1 was downregulated ((b)(III)).
Moreover, keratin 1 as an associated protein with Hsp74 was found by coimmunoprecipitation.
Identified Keratin 1. Keratin 1 (Figure 4) was identified associated with Hsp74 by coimmunoprecipitation (Figure 3), LC-MS/MS.
Hsp74 was associated with keratin 1 as determined by coimmunoprecipitation from bladder cancer cell line BLZ211.
In Figure 6 we compare the spectra of keratin, multiwalled carbon nanotubes, multiwalled carbon nanotubes oxidized, and three samples of experiments at 75[degrees]C with MWOHs/keratin 1:1wt/wt (CK7511), 65[degrees]C with MWOHs/ keratin 1
: 1 wt/wt (CK6511), and 55[degrees]C with MWOHs/keratin 1: 3 wt/wt (CK5513).
A mutation in the V1 end domain of keratin 1
causing non-epidermolytic palmar-plantar keratoderma.