Se estudiaron 31 genes de queratina reportados para ovinos, cabras y humanos entre los cuales tenemos 12 genes del epitelio de tipo I (KRT9, KRT10, KRT12, KRT13, KRT14, KRT15, KRT16, KRT17, KRT18, KRT19
, KRT20 y KRT25), 13 genes del epitelio de tipo II (KRT1, KRT2, KRT3, KRT4, KRT5, KRT6a, KRT6b, KRT6c, KRT7, KRT8, KRT71, KRT79 y KRT80), 3 genes de la fibra de tipo I (KRT31, KRT32 y KRT40) y 3 genes de la fibra de tipo II (KRT81, KRT82 y KRT83) (Zhidong et al.
We first developed and analytically validated 3 multiplex RT-qPCR assays for the quantitative determination of (a) Epithelial markers: CK-19 (KRT19
), EpCAM, E-cadherin (CDH1), and HMBS (reference gene); (b) Stem cell markers: PSCA, ALDH1A1, CD133 (PROM1), and HPRT1 (reference gene); and (c) EMT markers: TWIST1, Vimentin (VIM), N-cadherin (CDH2), and B2M (reference gene).
3630 INS Identical protein binding and protease binding 4316 MMP7 Peptidase activity and metallopeptidase activity 3880 KRT19
Structural molecule activity and structural constituent of cytoskeleton 7057 THBS1 Calcium ion binding and heparin binding 3320 HSP90AA1 Poly(A) RNA binding and identical protein binding 1508 CTSB Peptidase activity and cysteine-type peptidase activity 7076 TIMP1 Cytokine activity and protease binding.
Normal versus tumor biomarkers LN negative versus LN positive biomarkers SLC39A5, C3orf32, TP73, CD1B, LAT2, TTC13, ARV1, NMT1, PCDHGA4, PCDHGA11, PCDHGA9, DCAKD, GJA1, OR7A17, LOX, PCDHGA1, PCDHGB1, PCDHGB6, KRT19
, ZNF655, KRTAP4-4, PCDHGA12, PCDHGB3, PCDHGB7, TAAR5, SEMA3E, HOXD1 PCDHGA6, PCDHGA8, PCDHGA10, PCDHGA5, PCDHGB4, PCDHGA3, PCDHGA2, PCDHGB2, PCDHGA7, PCDHGB5, C20orf197, SLC16A5, FUT8, SLC15A2, C17orf93, PRAC, OCLN, TMEM144, FGF2, PDX1, CCL1, LILRB5, LCE3D, GPR45, LPO, CGB5
Zhang et al., "A novel biomarker Linc00974 interacting with KRT19
promotes proliferation and metastasis in hepatocellular carcinoma," Cell Death and Disease, vol.
The combination of GDF5 gene transfer and 3D culture in alginate showed a significant upregulation of SOX9 as a marker for chondrogenesis and KRT19
as a marker for discogenesis compared to untransfected cells (Figure 3).
In contrast, genes involved in stem cell maintenance, such as cell adhesion molecules, WNT, and Notch-signaling pathway components, CDH1, SERPINF1, LEF1, FRZB1, KRT19
, SOD2, and EGR1, were overexpressed in the limbal crypt .
Each sample was profiled in duplicates for expression of TUBB2A,  TBP, GUSB, GAPDH, EMP2, VIM, VEGFA, PLAU, TWIST1, TP53, TOP2A, SCGB2A2, SATB1, RAD51, PTPRC, PTEN, PIK3CA, PGR, PARP1, MYC, MUC1, MTOR, ABCC1, MET, KRT19
, KRAS, KIT,MKI67, IGF1R, IBSP, FLT1, ESR1, ERBB2, EPCAM, EGFR, CTSD, CDH1, CD44, CD24, AURAK, ALDH1A1, AKT2, ADAM17, ACTB (Actin fi), PPIA, and B2M.
The network and GO analysis (Table 3) showed that proteins such as EpCAM, FER, SYK, CDH1, KRT8, KRT19
, and KRT18 are associated with cell adhesion; hormones such as POMC, IAPP, PTHLH, and ACE are associated with cell metabolism; CXCL5, CCR7, CD55, CD46, and CD47 are associated with immune activation; and TF, FN1, ADAMTS4, APCS, CTSD, and MMP13 are associated with extracellular matrix remodeling.
Moreover, the proteome in the present study included several laryngeal cancer-associated blood markers identified in previous studies, such as CAT, IL6, IL8, S100A9, PFN1, HSPA1A, MMP2, MMP3, KRT19
, SERPINB3, PRDX3, and LGALS3BP These results verified the quality of the laryngeal cancer-derived "secretory/releasing" protein database in uncovering novel biomarkers for this disease.
Quantitative real-time PCR (qPCR) targeting keratin19 mRNA (KRT19
)  has been widely used to detect and monitor CTCs in cancer patients (5, 6).
These genes encode components of desmosomes (DSG2), gap junctions (CX26), tight junctions (CLDN4, CLDN7), the cornified envelope (SPRRIA, 2A, 2B, 2E, 2F, 2G, 2I, 2J), intermediate filaments (KRT19
), and a variety of cell-surface and extracellular-matrix glycoproteins (SPP1, BGP1, BGP2, MUC1, TROP2, CLU).