Se estudiaron 31 genes de queratina reportados para ovinos, cabras y humanos entre los cuales tenemos 12 genes del epitelio de tipo I (KRT9, KRT10, KRT12, KRT13, KRT14, KRT15, KRT16, KRT17, KRT18, KRT19, KRT20 y KRT25), 13 genes del epitelio de tipo II (KRT1, KRT2, KRT3, KRT4, KRT5, KRT6a, KRT6b, KRT6c, KRT7, KRT8, KRT71, KRT79 y KRT80), 3 genes de la fibra de tipo I (KRT31, KRT32 y KRT40) y 3 genes de la fibra de tipo II (KRT81, KRT82 y KRT83) (Zhidong et al.
Se identificaron un total de 48 PNSs de los cuales 14 PNSs se encuentran distribuidos en 8 genes de queratina de epitelio de tipo I (KRT9, KRT12, KRT13, KRT14, KRT16, KRT18, KRT20, KRT25); 23 PNSs distribuidos en los 8 genes de queratina de epitelio de tipo II (KRT1, KRT3, KRT5, KRT6a, KRT7, KRT8, KRT71, KRT80); 4 PNSs en los genes de queratina de fibra de tipo I (KRT31, KRT32, KIRT40) y 7 PNSs en los genes de queratina de fibra de tipo II (KRT81, KRT82, KRT83).
Further, keratin 18 (KRT18
) levels were decreased by both TPP and IPTP; however, the expression of this gene was not changed in response to troglitazone (Figure 5K; see also Figure S3).
Furthermore, in [Ogg1.sup.-/-] HCCs, significant overexpression of keratins 8 (KRT8) and 18 (KRT18), prohibitin 1 (PHB1), calreticulin (CALR), and histidine triad nucleotide-binding protein 1 (HINT1) transcriptional factors, vimentin (VIM), chitinase-like 3 (Chil3), serine dehydratase (SDS), methionine adenosyltransferase 1A (MAT1A), and PGRMC1 was detected.
Significant elevation of proteins inducible by oxidative stress, which could participate in progression of HCA to HCC, such as KRT8, KRT18, PHB1, CALR, and VIM, was observed in the [Ogg1.sup.-/-] mouse HCCs.
The network and GO analysis (Table 3) showed that proteins such as EpCAM, FER, SYK, CDH1, KRT8, KRT19, and KRT18
are associated with cell adhesion; hormones such as POMC, IAPP, PTHLH, and ACE are associated with cell metabolism; CXCL5, CCR7, CD55, CD46, and CD47 are associated with immune activation; and TF, FN1, ADAMTS4, APCS, CTSD, and MMP13 are associated with extracellular matrix remodeling.
Studies show that only LY lines can express keratin 8 (Krt8) and keratin 18 (Krt18
) which are found in non-stratified epithelia but not in keratinocytes .
Genes APE ESE KJ Expected sum ACOT7 -1.586 -1.516 -1.723 -3.102 APOBEC3F -1.444 -1.558 -1.647 -3.002 APOBEC3G -1.619 -1.376 -1.705 -2.995 ASS1 -1.630 -1.366 -1.834 -2.996 CXCL12 1.564 -7.500 7.597 0.064 F2RL2 2.211 1.454 2.594 3.665 GBP1 -1.729 -1.464 -1.636 -3.193 GLDN 1.569 1.464 1.772 3.033 H1ST1H2AC -1.765 -1.424 -1.558 -3.189 KRT18
-1.796 -1.591 -2.166 -3.387 LYPD1 -1.699 -1.464 -1.591 -3.163 NAMPT 1.860 -7.647 2.757 0.213 PSMB8 -1.597 -1.495 -1.439 -3.092 RCAN1 -1.647 -1.376 -1.444 -3.023 RNY4 2.092 1.361 1.510 3.453 SLC6A1 1.449 1.905 1.586 3.354 SLC02B1 2.990 1.449 3.238 4.439 SRXN1 2.063 1.361 1.952 3.424
Using one-step real-time reverse transcriptase-PCR, we have further validated the pregnancy association of 5 newly identified transcripts, which were abundant in predelivery maternal plasma, i.e., signal transducer and activator of transcription 1,91kDa (STAT1), guanylate binding protein 1, interferon-inducible (GBP1), and hydroxysteroid (17beta) dehydrogenase 1 (HSD17B1) in 10 additional plasma samples from third-trimester pregnant women, as well as keratin 18 (KRT18) and growth arrest and DNA-damage-inducible, gamma (GADD45G) in 10 plasma samples from another cohort of third-trimester pregnant women (see online Supplemental Fig.
 Human genes: PAPPA, pregnancy-associated plasma protein A, pappalysin 1; H19, H19, imprinted maternally expressed transcript (non-protein coding); STAT1, signal transducer and activator of transcription 1, 91kDa; GBP1, guanylate binding protein 1, interferon-inducible; HSD17B1, hydroxysteroid (17-beta) dehydrogenase 1; KRT18, keratin 18; GADD45G, growth arrest and DNA-damage-inducible, gamma.
The relationships of EPOR (erythropoietin receptor), MAPK14 (mitogen-activated protein kinase 1), BCL2L1 (BCL2-like1), KRT18
(keratin 18), PTPN6 (protein tyrosine phosphatase nonreceptor 6), CASP3 (caspase-3), TGFBR2 (transforming growth factor-beta, TGF[beta], type II receptor), AR (adrenergic receptor), and CASP7 (caspase-7) with gastric cancer were already known.
KRT13 was reported to occur in combination with KRT8, KRT18
and KRT20 in UCB .