Apart from them, transcription factors including CREB, Krox20
, KLF5, and STAT5 participate in the complex network of transcription regulation .
Milbrandt, "NAB2, a corepressor of NGFI-A (Egr-1) and Krox20
, is induced by proliferative and differentiative stimuli," Molecular and Cellular Biology, vol.
(1) These hair shaft progenitors express transcription factor KROX20
, which expresses stem cell growth factor necessary for hair pigmentation by maintenance of differentiated melanocytes.
Figures 4(a)-4(d) show the immunostaining results of Krox20
proteins, which stained as red, while the nucleus stained as blue, indicating that the expression of Krox-20 was increased after EGCG addition.
Ambos estados y el proceso de cambio entre ambos es regulado por factores reguladores intrinsecos celulares: unos positivos, como Krox20
, Sox10 y Oct6, los cuales aseguran la mielinizacion de nervios el tiempo adecuado durante el desarrollo y restablecen la funcion de las celulas de Schwann despues de la lesion, y otros negativos, como c-Jun, Sox2, Notch, Krox24 y Pax3, los cuales conducen al proceso de dediferenciacion y desmielinizacion, al bloquear la normal induccion de mielinizacion por parte de la adenosina monofostato ciclico.
Remyelination is another important process involved in nerve regeneration whereby Schwann cells are able to flourish due to myelination and the inhibition of [H.sub.2]S production (via transcriptional regulation through krox20
and c-jun), which is fundamental to this stage of regeneration .
Here, we examine whether GGF-2 is sufficient alone to maintain critical Schwann-like properties by examining dASCs ability to maintain a Schwann-like morphology, produce brain-derived neurotrophic factor (BDNF), and express Krox20 (the main transcription factor involved in myelination) .
Primer sequences are Krox20 [forward sequence: GGAGTGGCCGGAGAT-3, reverse sequence: GAGATCCAACGACCTCT T-3' (Sigma-Aldrich)], 18s [Qiagen (Cat number PPH05666E-200)] and BDNF [Qiagen (Cat number PPH00569F-200)].
[dASC.sup.-/+GGF] upregulated Krox20 expression compared to [dASC.sup.+] (Figure 3(a)) but failed to maintain BDNF production levels regardless of GGF-2 concentration (Figure 3(b)).
We investigated whether a similar effect would be seen in dASCs by stimulating cells with GGF-2 at increasing concentrations and quantifying Krox20 expression.
Finally, they identified that GGF-2 alone supports the expression of Krox20
, a transcription factor indispensable for myelination.