EZH2 knockdown upregulates miR-30a expression that directly increased miR-30a target KPNB1, decreasing malignant peripheral nerve sheath tumor cell proliferation .
Additionally, downexpression of EZH2 upregulates miR-30a expression and miR-30a overexpression directly inhibits its target KPNB1, inducing cell apoptosis.
Ma et al., "Antitumor effects of pharmacological EZH2 inhibition on malignant peripheral nerve sheath tumor through the miR-30a and KPNB1 pathway," Molecular Cancer, vol.
Similarly, galectin-1 (Lgals1) appeared to be represented by three clones; karyopherin [beta]1 (Kpnb1) and H3 histone 3B (H3f3b) were represented by two clones each.
The microarray analysis appeared to underestimate the fold change of expression compared with qPCR (Figure 3), with the only exception being Kpnb1, for which a downregulation in embryos was not supported by qPCR (Figure 3).
Although Kpnb1 is a gene whose expression has been reported to be downregulated by HDAC inhibition (Marks et al.