PPAR[delta] activates PPRE-containing genes associated with metabolism (Olah, Ptgs2, Pla2, and Pld), invasion (Mmp12, Klk6
), and inflammation (S100a8/9, Saa1/2/3).
TaqMan gene expression assays used were Hs00160519_m1 (kallikrein 6, KLK6
), Hs00173611_m1 (kallikrein 10, KLK10), Hs00234579_m1 (matrix metalloproteinase 9, MMP9) and Hs99999905_m1 (glyceraldehyde 3-phosphate dehydrogenase, GAPDH).
A list of candidate biomarkers was chosen according to a set of criteria, highlighting KLK6
, a protein that belongs to the 15-member KLK family in which KLK3 (also known as prostate-specific antigen) is the most useful marker for the early detection of prostate cancer.
, the protein encoded by the Kallikrein-related peptidase 6 (KLK6
)  gene, is a promising biomarker that is frequently overexpressed at the mRNA and protein levels in tissues and fluids derived from patients with ovarian cancer (OCa) and implicated in a variety of normal physiological processes (3).
Scarisbrick previously found elevated levels of "KLK6
" (a newly identified member of the kallikrein enzyme family) in areas of damage found in tissue samples from people with MS.
These genes include angiogenic/vascular cell growth factors (VEGF, PLGF, ADM, ANGPT2, TGFB2), vasoactive serine proteases (KLK2, KLK6
, KLK9, KLK22), and vascular endothelial receptors (IL17R, BDKRB1, ENG, GNA13).
The amounts of 18S rRNA and mRNAs encoded by KLK5, KLK6
, KLK7, KLK10,
Recent work has demonstrated roles for KLK5 and 7 in skin desquamation (7), and for KLK6
in the brain (8).
(30) found no overexpression of the 15 tissue kallikreins in breast cancer tissue compared with healthy breast tissue and down-regulation of the expression of at least 4 tissue kallikrein genes (KLK5, KLK6
, KLK8, and KLK10) in breast cancer tissue.
For example, we found that KLK6
and KLK13 have a splice variant with 3'-coding exon 3 extension by ~100 by (close to the length of the retained intron III in the genes examined here).
The human kallikrein 6 (KLK6
) gene, which encodes for hK6 protein, was cloned independently by three groups.