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A recent case-control study based on the LC-MS/MS and iTRAQ found 50 altered proteins (39 overexpressed and 11 subexpressed); three of them were validated through ELISA: alpha-enolase, IL-18, and KLK13 .
controls BMS No significant differences BMS No significant differences BMS [up arrow] Alpha-enolase, [up arrow] IL-18, and [up arrow] KLK13 in BMS vs.
KLK11, KLK13, and KLK14 were measured as previously described (18, 19) (see Supplemental Data section in the online Data Supplement).
The KLK8 mRNAs were considered alone or as a ratio with the KLK5, KLK6, KLK7, KLK10, KLK11, KLK13, or KLK14 mRNA in the same samples.
Dore, "KLK6 and KLK13 predict tumor recurrence in epithelial ovarian carcinoma," British Journal of Cancer, vol.
KLK13 was found at high concentrations in the esophagus and tonsil.
KLK13 was found at highest concentrations in CVF, followed by seminal plasma and saliva.
For example, we found that KLK6 and KLK13 have a splice variant with 3'-coding exon 3 extension by ~100 by (close to the length of the retained intron III in the genes examined here).
KLK13, previously known as KLK-L4, is one of the newly identified kallikrein genes.
CLONING OF KLK13 CDNA INTO THE YEAST EXPRESSION SYSTEM
A noteworthy pattern related to the hormonal regulation is that the centromeric and telomeric groups of kallikreins (KLK1 to -4 and KLK13 to -15) are up-regulated mainly by androgens, whereas the central group is up-regulated mainly by estrogens.
New gene Previous gene New protein symbol (a,b) symbol(s) symbol KLK1 KLK1 hK1 KLK3 KLK3 hK3 KLK2 KLK2 hK2 KLK4 PRSS17, KLK-L1, KLK4 hK4 KLK5 KLK-L2 hK5 KLK6 PRSS9 hK6 KLK7 PRSS6 hK7 KLKS PRSS19 hK8 KLK9 KLK-L3 hK9 KLK10 PRSSL1, hK10 KLK11 PRSS20 hK11 KLK12 KLK-L5 hK12 KLK13 KLK-L4 hK13 KLK14 KLK-L6 hK14 New gene symbol (a,b) Other protein names/symbols KLK1 Pancreatic/renal kallikrein, hPRK KLK3 Prostate-specific antigen, PSA KLK2 Human glandular kallikrein 1, hGK-1 KLK4 Prostase, KLK-L1 protein, EMSP1 KLK5 KLK-L2 protein, HSCTE KLK6 Zyme, protease M, neurosin KLK7 HSCCE KLKS Neuropsin, ovasin, TADG-14 KLK9 KLK-L3 protein KLK10 NES1 protein KLK11 TLSP/hippostasin KLK12 KLK-L5 protein KLK13 KLK-L4 protein KLK14 KLK-L6 protein New gene GenBank symbol (a,b) Accession No.
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