KLF7 Has a Transcriptional Activation Effect on IL-6.
To clarify the KLF7 and IL-6 promoter-specific binding sites, the truncated luciferase reporter plasmid (1501 bp, 1001 bp, and 501 bp) of the core segment of the human IL-6 promoter (length 2001 bp) was further constructed.
KLF7 plays an important regulatory role in lipid and glucose metabolism.
Our previous experimental results suggest that the mRNA and protein expression levels of KLF7, the subunit p65 of NF-[kappa]B, and the downstream inflammatory cytokine IL-6 in visceral adipose tissue were significantly higher in obese, compared with normal weight, individuals.
Our previous experimental results suggest a significant positive correlation between the TLR4 and KLF7 expressions in visceral adipose tissue of obese individuals.
On the contrary, Chen et al.'s study reported that human obese individuals have lower KLF7 expression .
Next, we evaluated the effect of KLF7 on neurite outgrowth of DRG explants using the same imaging techniques (Figure 4(e)).
KLF7 Expression Is Dynamically Altered following SCI.
AAV-KLF7 Significantly Increases the Expression of KLF7 and Its Downstream Targets and Mitigates Injury-Related Loss of the Myelination Protein following SCI.
To test the effect of AAV-KLF7 on the expression of KLF7 target genes in vivo, we examined the expression of NGF and TrkA, the axon regeneration marker GAP43, and the myelinated axon marker P0 in thoracic spinal cord tissues (Figure 6(a)).
However, we found that KLF7 expression and thus the percentage of FG/KLF7 double-labeled neurons in the population were higher in the SCI + AAV-KLF7 group compared to the SCI + AAV-GFP group (KLF7: SCI + AAV-KLF7, 2971 [+ or -] 701.1; SCI + AAVGFP, 195.1 [+ or -] 101.5; [t.sub.10] = 9.598, P < 0.001; percentage of FG/ KLF7 neurons: SCI + AAV-KLF7, 90.75 [+ or -] 3.84%; SCI + AAV-GFP, 3.11 [+ or -] 1.54%; [t.sub.10] = 51.83, P <0.001) (Figures 8(c) and 8(e)).
These results demonstrated that KLF7 upregulation enhances DPST plasticity at the caudal spinal cord following SCI.