KDM4B

KDM4B

A gene on chromosome 19p13.3 that encodes lysine-specific demethylase 4B, which demethylates histone H3; it generates formaldehyde and succinate and acts as a transcription repressor.
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The striking features of micropapillary UC were downregulation of miR-296, with overexpression of its nearly 300 target genes and activation of chromatin-remodeling complex RuvB-like 1 (RUVBL1) with overexpression of its downstream target genes such as lysine-specific demethylase 4B (KDM4B), insulin-like growth factor-binding protein 3 (IGFBP3), and disintegrin and metalloproteinase domain-containing protein 15 (ADAM15) involved in cell growth, DNA damage repair, and metastasis.
Yu et al., "Histone demethylases KDM4B and KDM6B promotes osteogenic differentiation of human MSCs," Cell Stem Cell, vol.
Of the genes that span this region, KDM4B has been found in murine models to be active during embryogenesis, specifically within the visual system [19].
The histone methyltransferases (SET1, G9a, SUV39H1, and SUV39H2) and the histone demethylases (LSD1, KDM3A, KDM4A, KDM4B, KDM4C, KDM5A, KDM5B, KDM5C, and KDM5D) gene expression was detected by real-time quantitative PCR.
In order to investigate the cause of altered histone methylation patterns in LADA patients, we assessed mRNA levels of histone methyltransferases (SET1, G9a, SUV39H1, and SUV39H2) and the histone demethylases (LSD1, KDM3A, KDM4A, KDM4B, KDM4C, KDM5A, KDM5B, KDM5C, and KDM5D) in [CD4.sup.+] T lymphocytes by real-time quantitative PCR.
Histone 3 lysine 9 methylation was regulated by histone methyltransferase G9a, SUV39H1, SUV39H2, and histone demethylase LSD1, KDM3A, KDM3B, KDM4A, KDM4B, and KDM4C [20, 21].
The mRNA level of histone methyltransferases gene (SET1, SUV39H1, SUV39H2, and G9a) and histone demethylases gene (LSD1, KDM3A, KDM4A, KDM4B, KDM4C, KDM5A, KDM5B, KDM5C, and KDM5D) was detected by real-time PCR.
(b) Relative mRNA levels of histone demethylases (LSD1, KDM3A, KDM4A, KDM4B, KDM4C, KDM5A, KDM5B, KDM5C, and KDM5D) in [CD4.sup.+] T lymphocytes from LADA patients (n = 26) and healthy controls (n = 26), the absolute of fold change > 2 was considered significant, LADA patients versus healthy controls; data are normalized against beta-actin.
He and his team found that KDM4B and KDM6B, two gene-activating enzymes, can promote stem cells' differentiation into bone cells by removing methyl markers from histone proteins.
The research group, through its study of aging mice, found that the two enzymes KDM4B and KDM6B could specifically activate genes that promote stem cell differentiation toward hone, while blocking the route toward fat.
"Interestingly, in our aged mice, as well as osteoporotic mice, we observed a higher amount of silencing histone methyl groups which were normally removed by the enzymes KDM4B and KDM6B in young and healthier mice," Wang said.
Citation: "Histone Demethylases KDM4B and KDM6B Promotes Osteogenic Differentiation of Human MSCs;" Ling Ye et al.; Cell Stem Cell, 2012; 11{1): 50 DOI: 10.1016/j.stem.2012.04.009