Another study proved by Kuang and coworkers  revealed that EZH2 expression was positively correlated to KDM2B, which controls gene expression by the demethylation of dimethyl histone H3 lysine 36 (H3K36me2) and trimethyl histone H3 lysine 4 (H3K4me3).
Guo et al., "Histone demethylase KDM2B upregulates histone methyltransferase EZH2 expression and contributes to the progression of ovarian cancer in vitro and in vivo," OncoTargets and Therapy, vol.
The mRNA expression levels of KDM2A, KDM2B, and EZH2 were higher in TNF-[alpha]-treated cells compared to control cells, and AZM mitigated this effect (Figure 3(g)-3(i)).
The histone lysine demethylases (KDMs) KDM2A and KDM2B demethylate H3K4me3 and H3K36me1/2 .
KDM2B is a component of the noncanonical PRC1 (polycomb repressive complex 1), and it recruits Ring1B and Nspc1 to promote H2AK119 monoubiquitylation .
KDM2B localizes to regions where H3K36me2 levels are low.
Abbreviations BSP: Bone sialoprotein EZH2: Enhancer of zeste homolog 2 FBS: Fetal bovine serum FITC: Fluorescein isothiocyanate KDM2A: Lysine-specific demethylase 2A KDM2B: Lysine-specific demethylase 2B OCN: Osteocalcin PI: Propidium iodide Runx2: Runt-related transcription factor 2.