The keratin-associated protein 8.1 (KAP8.1) is one member of the HGT proteins that belong to one of the three classes of KAP proteins (Kuczek and Rogers, 1987; Fratini et al., 1992; Aoki et al., 1997).
PCR was performed to amplify sequences fragments of KAP8.1. Three pairs of PCR primers (Table 1) were designed using the software Oligo 6.0, according to the mRNA sequence of KAP8.1 (GenBank accession no.
The nucleotide sequence analyses of the six genotypes revealed that three different alleles, A (G-G), B (T-G) and C (G-C), which contained two SNPs (Figure 2) were identified in the KAP8.1 mRNA sequence (GenBank accession no.
Cashmere traits least square means for KAP8.1 T113G genotypes are same as in Table 3.
In the current study, six genotypes and three alleles were identified by SSCP (Figure 1), sequencing showed that two mutations of the KAP8.1 gene, which supports previous research (Zhao et al., 2009).
The current results are the first to reveal that the polymorphisms of KAP8.1 are associated with cashmere weight, cashmere length and hair length (Table 3).
The SNPs in the KAP8.1 gene in this study were all synonymous mutations and are therefore traditionally viewed as being phenotypically silent since they do not alter the amino acid sequence of the subsequent protein.
In conclusion, three alleles and six genotypes of the KAP8.1 gene were identified in Inner Mongolia Cashmere goats.