H+

(redirected from K+-ATPase)

H+

Symbol for hydrogen ion, the proton.

H+

Abbreviation for hydrogen ion, the proton.
References in periodicals archive ?
Methods: Enzymatic assays, measuring the amount of inorganic phosphate produced, were used to estimate the activity of Na+, K+-ATPase.
Results: The results show that Porphyrin compounds exert an insulin-like effect on Na+, K+-ATPase. They act by increasing the activity of the membrane-bound enzyme.
Conclusion: All the three Porphyrin compounds increased the activity of erythrocyte Na+, K+-ATPase. The exact mechanism of action of these compounds is not clear.
KEY WORDS: Na+, K+-ATPase, Porphyrin derivatives, Diabetes Mellitus, Acetylcholinesterase, Dysnatremias.
Na+, K+-ATPase pumps Na+ and K+ ions across the cell membranes to maintain the transmembrane gradients of these ions.
They possess specific receptors for insulin.8 It is known that insulin deficiency during Diabetes Mellitus causes membrane alterations in erythrocytes.9 A decreased activity of erythrocyte membrane Na+, K+-ATPase has been reported in Diabetics.10 This decreased activity plays a major role in the development of cardiomyopathy.11 and may also result in metabolic, cardiovascular, ocular, and neural complications.12 Some studies have previously shown that Na+, K+-ATPase activity is decreased in erythrocytes during Diabetes Mellitus.13 Diabetic patients show a 30% reduction in erythrocyte Na+, K+-ATPase activity as compared to controls.
We have previously evaluated Porphyrins in the context of photodynamic therapy (PDT) since they strongly absorb visible light in the blue region.14 In addition, we have also shown that these compounds serve as inhibitors for Acetylcholinesterase enzyme in erythrocytes and nerve tissue.15,16 This study was conducted to investigate the three Porphyrin derivatives: Tetraphenylporphinesulfonate (TPPS), 5,10,15,20-Tetrakis (4sulfonatophenyl) porphyrinato Iron(III) Chloride (FeTPPS) and 5,10,15,20-Tetrakis (4sulfonatophenyl) porphyrinato Iron(III) nitrosyl Chloride (FeNOTPPS), as candidate compounds for increasing the Na+, K+-ATPase activity in erythrocytes.
Determination of Na+, K+-ATPase activity: The enzymatic activity of Na+, K+-ATPase was measured using published protocols.20 The amount of inorganic phosphate produced was estimated by the method of Fiske and Subbarow.21
Wyse, "Dietary soy prevents brain Na+, K+-ATPase reduction in streptozotocin diabetic rats," Diabetes Research and Clinical Practice, vol.
ATPase and phosphatase activity of Na+, K+-ATPase: molar and specific activity, protein determination.
Change in the activity of Na+, K+-ATPase in embryos of the sea urchin, Hemicentrotus pulcherrimus, during early development.
Expression of Na+, K+-ATPase [alpha]s-subunit in animalized and vegetalized embryos of the sea urchin, Hemicentrotus pulcherrimus.