Transduction was performed according to the manufacturer's protocol, as previously described. The transduction efficiencies of the LVs in Jurkat cells
were monitored by fluorescence microscopy 3 days after transduction.
The results of 27 MHz quartz-crystal microbalance (QCM) measurements of DR5 with 5 and 6 and costaining experiments of Jurkat cells
with 5 and anti-DR5 antibody that 5 and 6 bind to DR5 at the different sites from the recognition sites of anti-DR5 antibody.
Simian retrovirus (SRV) infection of Jurkat T lymphocytes (Jurkat cells
) was confirmed by syncytia formation, of which the membrane of the neighboring cells fused to one another.
Briefly, the unactivated and activated Jurkat cells
(1 x [10.sup.6] cells/well) were treated in triplicate with, or without, Ti(IV) ions (5, 25, 50, or 100 [micro]M) in 6-well plates for 24 h.
Methods: Flow cytometry (FCM) analyses of Jurkat cells
treated with CSM bark extract (0-500 ugxmL-1) for 24-72 h allowed evaluating its cytotoxicity and ability to induce apoptosis through the intrinsic or extrinsic pathways.
Role of microRNA-143 in Fas-mediated apoptosis in human T-cell leukemia Jurkat cells
. Leuk Res 2009; 33: 1530-1538, doi: 10.1016/j.leukres.
Suzuki-Karasaki, "Mitochondrial superoxide mediates mitochondrial and endoplasmic reticulum dysfunctions in TRAIL-induced apoptosis in Jurkat cells
," Free Radical Biology & Medicine, vol.
The CD30-aptamer PEI siRNA nanoplex bound selectively to Karpas 299 cells (an ALCL line), whereas this targeted nanoplex showed little binding to Jurkat cells
, a cell line that does not have the CD30 surface marker.
Wachi, "Generation of hydrogen peroxide primarily contributes to the induction of Fe(II)-dependent apoptosis in Jurkat cells
by (-)-epigallocatechin gallate," Carcinogenesis, vol.
An earlier study found that, with the inhibition of calcium influx, CaMKII could be activated by ROS in Jurkat cells
which had been pretreated with hydrogen peroxide .
Tomoda, "Fungal malformins inhibit bleomycin-induced G2 checkpoint in Jurkat cells
," Biological and Pharmaceutical Bulletin, vol.