To monitor mitochondrial function, Jurkat cells
were irradiated (0 or 10 Gy) and further cultured for 6 h.
Exposure of human p53 mutant Jurkat cells
to DNA-damaging agents activates arrest in the G2 cell cycle phase at the G2/M DNA damage checkpoint and attempts homologous recombination repair (Seifrtova et al.
50] values indicated that ESV was more effective in Jurkat cells
than in the erythroleukemic cell line K562 ([IC.
1] and allowed to adhere in 96 well plates for a minimum of 4 h In parallel U937 and Jurkat cells
were also seeded and different CPT extracts were added to all the cells at the same time and they were cultured further for 48 h.
In addition, mistletoe extract seemed to partially protect healthy PBMC - but not malignant Jurkat cells
- from the cytostatic effect of 4-hpc.
were resuspended in 350[micro]1 and transfected by electroporation using an Easyject plus Electroporator (Equibio, Middlesex, UK).
The cytotoxic and anti-proliferative effects of 3-hydrogenkwa-daphnin in K562 and jurkat cells
is reduced by guanosine.
Tumor necrosis factor-[alpha] (TNF-[alpha])-inducible inflammatory responses in HeLa and Jurkat cells
have been used extensively in the study of NF[kappa]B/I[kappa]B regulation (Beg and Baldwin, 1993).
We previously reported that okadaic acid, a tumor promoter mimics TNF-[alpha]/interleukin-l (IL-1), in inducing phosphorylat ion of the same proteins, and that TNF-[alpha] also induces similar expression of early-response genes in human fibroblasts, and activate NF-kB in Jurkat cells
(Guy et al.
Methods: We manipulated MFN2 gene expression in Jurkat cells
via lentiviral transduction of MFN2 small interfering RNA (siRNA) or full-length MFN2.
maritimum extract, is selective in inducing apoptosis specifically in Jurkat cells
(a human lymphoma cells) and in clinical leukemia samples with minimal effect on non-cancerous peripheral blood mononuclear cells.
Role of microRNA-143 in Fas-mediated apoptosis in human T-cell leukemia Jurkat cells