This is an important fact since the 2013 College of American Pathologists survey shows that 75% of laboratories in the US use a compensated Jaffe reaction
to measure creatinine.
The significance of this poisoning is that nitromethane interacts with the widely used Jaffe reaction method of determining plasma creatinine values.
When measured in the serum, this is balanced by the slightly higher value of creatinine due to nonspecific chromophores, which react with the colorimetric Jaffe reaction assay.
The plasma creatinine values were all measured by the Jaffe reaction, some of which were proven to be falsely high using the enzymatic method.
To correct for this, urinary creatinine was also assayed using a COBAS MIRA chemistry analyzer (Roche, Montclair, NJ; kinetic Jaffe reaction
In another study, serum creatinine was measured by a new modified kinetic Jaffe reaction and a modified version of the established enzymatic creatinine p-amino-phenazone (PAP) method to determine reference intervals for serum creatinine (83).
Interference by fluorescein with the single end-point determination of creatinine by use of the Jaffe reaction [Letter].
Deproteinization of serum: another best approach to eliminate all forms of bilirubin interference on serum creatinine by the kinetic Jaffe reaction. J Clin Lab Anals 2001;15:116-21.
Plasma and urinary Creatinine were measured by the Jaffe reaction
on an Hitachi 747 analyzer (Boehringer); 24-h Creatinine clearance was calculated from plasma and 24-h urinary Creatinine.
To counter these interferences, analysts have tried many variations of the Jaffe reaction, including modifications of sample preparation, addition of oxidizers to remove interferents, solvent extraction of interferents, pH adjustment of the reaction, continuous-flow dialysis, and kinetic measurement of the reaction [2-5].
The two Jaffe reactions were performed simultaneously with the same lot of reagents on different channels of the same Hitachi 747 analyzer.