Establishment of an immunoscreening system using recombinant VP1 protein for the isolation of a monoclonal antibody that blocks JC virus
Diagnostic confirmation of PML requires positive detection of JC virus
DNA within the CNS and characteristic CT or MRI imaging.
We thank Tiffany VanCleve, BS, (Department of Pathology, St Jude Children's Research Hospital) for her technical assistance with this project and Brent Seaton, PhD, (Focus Diagnostics, Cypress, California) for his support in providing control JC virus
Typing of urinary JC virus
DNA offers a novel means of tracing human migrations.
Polymerase chain reaction for the JC virus
has a sensitivity of 70%-90% and a specificity approaching 100%.
Despite some initial optimism that plasma JC viral DNA levels would be useful in detecting PML early, its sensitivity and positive predictive values are uncertain, and while CSF testing for JC virus
is specific at the time of diagnosis, the literature indicates that it tends to be negative in early disease and it is invasive; so it is not considered to be a good screening tool, he explained.
However, in many cases, confirmatory clinical symptoms, CSF polymerase chain reaction (PCR) studies that are positive for the JC virus
, and appropriate imaging findings may be sufficient for making a treatment decision, and biopsy can be avoided.
South Africa does have this technology, involving a polymerase chain reaction method to probe the presence of the JC virus
in the cerebrospinal fluid, and in the past 2 years the Department of Neurology at Steve Biko Academic Hospital in Pretoria has seen a rising number of patients with PML.
intranuclear inclusions associated with PML-NBs: analysis by electron microscopy and structured illumination microscopy.
A new laboratory test can detect antibodies to the JC virus
and help determine a person's risk of developing PML, a severe brain infection that has emerged in some people who have taken Tysabri.
In 1971, the JC virus
(JCV) was isolated from the brain of a patient with Hodgkin's disease who died of progressive multifocal leukoencephalopathy (PML).
A JC virus
(JCV) infection is required for the development of PML and the new label states that anti-JCV antibody negative status indicates that exposure to the JC virus
has not been detected.