A primary goal of the oocyte recovery technique is to maximize the total number of oocytes and the produce of high-quality oocytes recovered from each ovary (evenly granulated cytoplasm enclosed by numerous layers of dense CC), which can be used for IVM
, IVF, and in-vitro culture (IVC) processes (Behbahanian et al., 2013).
In both type of olfactometer a control treatment (80 g IVM-free feces) and experimental treatment (80 g feces from animals treated with 1 % IVM
) were used.
Contoversies in IVM
. J Assist Reprod Genet 2011;28:525-30.
Effects of low incubation temperature during IVM
on ICM, TE, and overall cell numbers
Essentially, the maturation stage of vitrified oocytes may impact the efficiency of the IVM
process (Fasano et al., 2010).
On the other hand, the coculture system in G1- and G2-PLUS media appeared to have detrimental effects on IVM
(Figures 1(b) and 1(c)) compared to the effects of culture in stock media.
Many couples drop out from IVF treatment due to the physical and psychological burden of conventional ovarian stimulation, and therefore, IVM
can provide for some couples a less intense option that avoids the bloating discomfort of conventional treatment.
This study evaluated the fate of IVM
MII oocytes subjected to three different culture mediums with subsequent embryo growth.
Effect of supplementation of IVM
medium with FCS, FAF-BSA and FAF-BSA+EGF on the cleavage rate and subsequent development of buffalo oocytes in vitro.
Muestras de leche de vaca y oveja sin residuos del farmaco fueron fortificadas con IVM
(1-10 ng/ml) y sometidas a tratamiento termico (pasteurizacion) a baja temperatura/largo tiempo (65[grados]C, 30 min, LTLT) o a alta temperatura/corto tiempo (75[grados]C, 15 s, HTST) en un bano termico de laboratorio.
Our assessment was based on information derived from two main sources: a) a qualitative external evaluation in May 2012 of IVM
implementation at two different geographic locations in Kenya between 2006 and 2011 (ICIPE 2012), and b) an analysis of entomological and malaria case data collected by the two projects.
Immature oocyte collection in a mild IVF program is still controversial for many IVF specialists and the impact of IVM
is decreased compared to conventional methods.