isoelectric focusing

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focusing

 [fo´kus-ing]
the act of converging at a point.
isoelectric focusing electrophoresis in which the protein mixture is subjected to an electric field in a gel medium in which a pH gradient has been established; each protein then migrates until it reaches the site at which the pH is equal to its isoelectric point.

i·so·e·lec·tric fo·cus·ing

electrophoresis of small molecules or macromolecules in a pH gradient.

isoelectric focusing

the ordering and concentration of substances according to their isoelectric points.

isoelectric focusing

the separation of molecules by differences in their charge, each molecule migrating to the point in a PH gradient where it has no net charge.

isoelectric focusing,

n the ordering and concentration of substances according to their isoelectric points.

focusing

the act of converging at a point.

isoelectric focusing
electrophoresis in which the protein mixture is subjected to an electric field in a gel medium in which a pH gradient has been established; each protein then migrates until it reaches the site at which the pH is equal to its isoelectric point.

isoelectric

showing no variation in electric potential.

isoelectric focusing
an electrophoretic method for separating proteins in gels which depends on the fact that the net charge on the protein varies with the pH of the surrounding medium. At the isoelectric point the protein has no net charge and will not therefore migrate further in an electric field. The polyacrylamide gel is cast in a narrow tube that provides a pH gradient from top to bottom.
isoelectric period
the moment in muscular contraction when no deflection of the galvanometer is produced.
References in periodicals archive ?
Analysis of the iron-binding sites of transferrin by isoelectric focussing.
This test involves separation of EPO idiotypes by isoelectric focussing, immunoblotting, and detection of IgG labeled with streptiverdin-peroxidase.
The different modes of capillary electrophoresis include capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), micellar electrokinetic capillary chromatography (MECC), isoelectric focussing and isotachophoresis (ITP).
Coverage includes electrophoretic techniques such as isoelectric focussing, SDS page, 2-D page, DIGE, liquid-chromatography techniques such as ion exchange, affinity chromatography or reversed-phase HPLC, and mass-spectrometric techniques such as MALDI, ESI, or FT ICR.