Isoelectric focussing: The isoelectric focusing was performed by harvesting 20 h brain infusion broth (Hi-media laboratories, India) culture by centrifugation.
Quality control for ESBL detection, isoelectric focussing and PCR: K.
Six randomly selected isolates (2 each from TEM, SHV and TEM+SHV positive groups) were subjected to isoelectric focussing. Results showed that TEM positive isolates produced beta lactamases consistent with pI (isoelectric point) of 5.4 and 5.6.
However, in certain instances it has proven beneficial to reduce the magnitude or even reverse the direction of the electroosmotic flow through the use of charged surfactants, which alter the zeta potential of the capillary inner walls.(12,13) Alternatively, for techniques like capillary isoelectric focussing
and capillary gel electrophoresis the electroosomotic flow is eliminated.
of this sample revealed several bands migrating between pI ~6 and 8.5.
Separation and identification of alkaline phosphatase isoenzymes and isoforms in serum of healthy persons by isoelectric focussing
. Clin Chem 1987;33:2171-7.