isoelectric focusing

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focusing

 [fo´kus-ing]
the act of converging at a point.
isoelectric focusing electrophoresis in which the protein mixture is subjected to an electric field in a gel medium in which a pH gradient has been established; each protein then migrates until it reaches the site at which the pH is equal to its isoelectric point.

i·so·e·lec·tric fo·cus·ing

electrophoresis of small molecules or macromolecules in a pH gradient.

isoelectric focusing

the ordering and concentration of substances according to their isoelectric points.

isoelectric focusing

the separation of molecules by differences in their charge, each molecule migrating to the point in a PH gradient where it has no net charge.

isoelectric focusing,

n the ordering and concentration of substances according to their isoelectric points.

focusing

the act of converging at a point.

isoelectric focusing
electrophoresis in which the protein mixture is subjected to an electric field in a gel medium in which a pH gradient has been established; each protein then migrates until it reaches the site at which the pH is equal to its isoelectric point.

isoelectric

showing no variation in electric potential.

isoelectric focusing
an electrophoretic method for separating proteins in gels which depends on the fact that the net charge on the protein varies with the pH of the surrounding medium. At the isoelectric point the protein has no net charge and will not therefore migrate further in an electric field. The polyacrylamide gel is cast in a narrow tube that provides a pH gradient from top to bottom.
isoelectric period
the moment in muscular contraction when no deflection of the galvanometer is produced.
References in periodicals archive ?
Quality control for ESBL detection, isoelectric focussing and PCR: K.
Six randomly selected isolates (2 each from TEM, SHV and TEM+SHV positive groups) were subjected to isoelectric focussing.
12,13) Alternatively, for techniques like capillary isoelectric focussing and capillary gel electrophoresis the electroosomotic flow is eliminated.
Isoelectric focussing of this sample revealed several bands migrating between pI ~6 and 8.
Separation and identification of alkaline phosphatase isoenzymes and isoforms in serum of healthy persons by isoelectric focussing.