isoelectric focusing


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Related to isoelectric focusing: isoelectric point

focusing

 [fo´kus-ing]
the act of converging at a point.
isoelectric focusing electrophoresis in which the protein mixture is subjected to an electric field in a gel medium in which a pH gradient has been established; each protein then migrates until it reaches the site at which the pH is equal to its isoelectric point.

i·so·e·lec·tric fo·cus·ing

electrophoresis of small molecules or macromolecules in a pH gradient.

isoelectric focusing

the ordering and concentration of substances according to their isoelectric points.

isoelectric focusing

the separation of molecules by differences in their charge, each molecule migrating to the point in a PH gradient where it has no net charge.

isoelectric focusing,

n the ordering and concentration of substances according to their isoelectric points.

focusing

the act of converging at a point.

isoelectric focusing
electrophoresis in which the protein mixture is subjected to an electric field in a gel medium in which a pH gradient has been established; each protein then migrates until it reaches the site at which the pH is equal to its isoelectric point.

isoelectric

showing no variation in electric potential.

isoelectric focusing
an electrophoretic method for separating proteins in gels which depends on the fact that the net charge on the protein varies with the pH of the surrounding medium. At the isoelectric point the protein has no net charge and will not therefore migrate further in an electric field. The polyacrylamide gel is cast in a narrow tube that provides a pH gradient from top to bottom.
isoelectric period
the moment in muscular contraction when no deflection of the galvanometer is produced.
References in periodicals archive ?
Isoelectric focusing of human hemoglobin: its application to screening, to the characterization of 70 variants, and to the study of modified fractions of normal hemoglobins.
Detection of oligoclonal IgG bands in CSF by isoelectric focusing is an important part of the diagnostic process in MS (Class I evidence).
Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues.
Louis, MO) was used to assay the soluble proteins in the egg protein solution and in the fractions after isoelectric focusing.
Isoelectric focusing (IEF) of muscle proteins has been the preferred method for identification of teleost fillets and products (Lundstrom, 1980; Rehbein, 1990); the method is used in legal cases to identify mislabeled fish products.
High resolution of peroxidase-indoleacetic acid oxidase isoenzymes from horseradish by isoelectric focusing.
Available as an upgrade module, the detector will also be integrated into four application-specific CE systems designed for automated and quantitative isoelectric focusing, oligosaccharide and monosaccharide analysis, DNA-protein interaction analysis, gene expression, and viral load determination.
Proteins from these two component blends and mixtures, as well as pure samples of each cultivar, were extracted and separated using isoelectric focusing and stained for esterase isozymes (Bell et al.
23-80, in Electrophoretic and isoelectric focusing techniques in fisheries management (D.
2]2-2 phenotypes in the Japanese by isoelectric focusing.
Conventional Isoelectric Focusing in Gel Slabs and Capillaries and Immobilized pH Gradients (Pier Giorgio Righetti, Alessia Farinazzo, Elisa Fasoli and Sabina Carla Righetti).
Contract notice: Supply, installation and maintenance of electrophoresis controllers (with integrated isoelectric focusing module).