Group Compound Extracted Pectase Alcohol 1-Propanol 0.53 [+ or -] 0.04 (a) Isobutyl alcohol 38.73 [+ or -] 0.65 (a) 1-Butanol 0.12 [+ or -] 0.01 (a) Isoamyl alcohol
153.59 [+ or -] 3.06 (a) 3-Ethoxy-1-propanol 0.16 [+ or -] 0.01 (a) 3-Hexen-1-ol 0.09 [+ or -] 0.01 (a) 2,3-Butanediol 0.07 [+ or -] 0.01 (a) alpha-Terpineol 0.19 [+ or -] 0.02 (a) 3-Methylthiopropanol 1.11 [+ or -] 0.13 (a) Benzyl alcohol 0.07 [+ or -] 0.00 (a) Phenylethyl alcohol 50.95 [+ or -] 4.56 (a) 3-Ethyl-1-butanol n.d.
A: phenol: chloroform: isoamyl alcohol
(25:24:1); B: phenol: chloroform (25:24)
Commercial ferment Natural ferment Parameter Non-aged Aged Non-aged Aged Fixed acidity (1) 1.14a 6.76b 1.13a 9.39c Volatile acidity (1) 3.29a 4.89b 3.27a 3.63ab Total acidity (1) 4.43a 11.65b 4.40a 13.02b pH 6.44b 4.70a 6.92b 4.27a Alcohol ([degrees]GL) 42.0a 40.2b 42.0a 40.5ab Copper (2) 0.39a 0.31a 0.44a 0.37a Acetaldehyde (2) 6.42ab 10.02b 5.62a 8.85ab Methanol (2) 9.31a 10.70a 8.50a 10.23a Ethyl acetate (2) 8.35a 10.63a 6.34a 8.84a N-propanol (2) 40.58a 50.99b 37.64a 49.35b Isobutanol (2) 45.50a 50.52b 36.26a 48.92b Isoamyl alcohol
(2) 131.01a 145.60a 120.83a 134.68a Higher alcohols (2) 217.08a 247.11b 194.73a 232.95b Total volatile compounds (2) 235.13ab 272.65b 209.97a 254.27b Different letters in the lines indicate significant difference (P<0.05 by Tukey's test).
Each eluate sample (5 mL) from the MBD column was concentrated with Amicon Ultra Centrifugal Filter Devices (30 000 MWCO, Millipore) to 200 [micro]L and then extracted with phenol/ chloroform/ isoamyl alcohol
(25: 24:1), precipitated in ethanol, and eluted in nuclease-free water.
Chloroform/ isoamyl alcohol
(24:1) and phenol/chloroform/ isoamyl alcohol
(25:24:1) extraction was performed consecutively and DNA was precipitated using 0.7 volumes isopropanol.
Then 50 uL of 3 M sodium acetate was added, followed by an equal volume of phenol: Chloroform: isoamyl alcohol
The straight-chain higher alcohols are considered to have the most significant sensorial impact (1-propanol, 2-methyl-1-propanol (isobutyl alcohol), 2-methyl-1-butanol and 3-methyl-1-butanol (isoamyl alcohol
Anhydrous iron(III) chloride (Fe[Cl.sub.3], Sd Fine Chemicals, India), ammonium hydroxide solution (30 w%, SRL, India), cyclohexane (SRL, India), isoamyl alcohol
(SRL, India), and Triton X-100 (SRL, India) were of analytical grade and used as received.
The supernatant was extracted with 750 [micro]L of chloroform: isoamyl alcohol
(24:1) by centrifugation at 15,000 rpm during 10 min to separate the phases.
This study investigates the effect of aging on the ability of Caenorhabditis elegans to chemotax from a point of origin to a known attractant, isoamyl alcohol
. After measuring the chemotaxis index of Caenorhabditis elegans aged three days, five days and seven days, we analyzed results to determine whether or not a statistically significant difference in the chemotaxis indices was present.
ABSTRACT: Synthesis of isoamyl salicylate was subjected to done by the reaction of isoamyl alcohol
with salicylic acid using different catalysts.