Interferon regulatory factor-1 as a positive regulator for high glucose-induced proliferation of vascular smooth muscle cells.
Interferon regulatory factor-1 together with reactive oxygen species promotes the acceleration of cell cycle progression by up-regulating the cyclin E and CDK2 genes during high glucose-induced proliferation of vascular smooth muscle cells.
The membranes were incubated with primary antibodies, including anti-T-bet/Tbx21antibody (1:1000), anti-Fas antibody (1: 1000) (Abcam, Cambridge, MA, USA), and anti-mouse-caspase-3, anti-mouse-cleaved caspase-3 antibody, anti-mouse-eukaryotic initiation factor 2 (eIF2) a (D7D3), anti-mouse-phospho-eIF2a (Ser51) (D9G8), anti-mouse-Fyn, anti-mouse interferon regulatory factor-1
(IRF-1), anti-mouse-signal transducer and activator of transcription (Stat) 1, and anti-mouse-Stat3 rabbit monoclonal antibodies (1: 1000, CST, Boston, MA, USA) and incubated with horseradish peroxidase-conjugated secondary antibody (CST).
Rockett et al., "Interferon regulatory factor-1
polymorphisms are associated with the control of Plasmodium falciparum infection," Genes & Immunity, vol.
IFN- a activates the transcription factor IRF-1 (interferon regulatory factor-1
), which also binds to elements in the NOS promoter [41,42].
IDO1 is induced by IFN-[gamma]-mediated effects of the signal transducer and activator of transcription 1[alpha] (STAT1[alpha]) and interferon regulatory factor-1
Wietzerbin, "Triggering of the human interleukin-6 gene by interferon-[gamma] and tumor necrosis factor-a in monocytic cells involves cooperation between interferon regulatory factor-1
, NF[kappa]B, and Sp1 transcription factors," The Journal of Biological Chemistry, vol.